Wbp

reconstruct_wbp

Purpose

This program allows you to generate 3D reconstructions from projections using the WeightedBackProjection algorithm with arbitrary geometry as described byRadermacher, M. (1992) Weighted back-projection methods. Electron Tomography, ed. by J. Frank, Plenum Press.

Usage

$ reconstruct_wbp ...

Parameters

• `` This parameter takes a selection file name which includes the projections to process
• `` This is the output filename for the volume
• -radius [floatdim2/] = The radius of the volume to be reconstructed. Outside this radius, the volume will be zero. The default radius is dim/2
• -threshold [float1.66] = Filter values below the threshold will be set to threshold. This is to avoid divisions by zero and consequent enhacement of noise. The threshold is given as a relative value with respect to the total number of images. The absolute threshold will be calculated internally as the relative threshold multiplied by the total number of (symmetry generated) projections. The default relative threshold value is 0.005
• -sym [symmetry file""] = If a symmetry file is given, both the filter and the reconstructed volume are symmetrized
• -filsam [float5] = Instead of summing over all experimental images to calculate the arbitrary geometry filter, we bin all images in representative projection directions, which are sampled every filsam degrees. The default sampling rate is 5 degrees
• `` If this option is given, all experimental images will be used in the summation to calculate the arbitrary geometry filter. For large datasets this may be considerably slower than the default option of using representative projection directions
• `` The shift of each image header is automatically applied when the image is read. Use this option to disable this feature

Examples and notes

$ reconstruct_wbp -i g1t.sel -o recons_wbp.vol

USER's COMMENTS

--Main.AlfredoSolano - 23 Jan 2007