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TiltPairPicker
Tilt Pair Picker allows you to pick particle pairs on tilted micrographs, either manually or automatically. It can be used in modes: manual, and readonly. Manual mode is used in the following way. First four particle pairs are selected manually. After this for each manual particle selected on the micrograph of reference tilted particle is picked automatically. Readonly mode allows to access tilpair picker data without edition enabled.
Tilt Pair Picker GUI its composed by a main window, two micrograph image windows and a particle pairs view. On main frame information for default family of particles is shown. Here color and size for particles are specified. Also micrographs information is displayed on a table (Figure 1).
Figure 1.Tilt Pair Picker Main Frame
From window menu particles view can be loaded. This view allows to see particles in detail, move them, and also remove it if necessary (Figure 2).
Figure 2.Particles View Frame
To improve image quality some filters are provided on the filters menu, and for a more advanced usage ImageJ can be loaded. On micrograph window particles can be added or dragged by using mouse left button. To remove a particle, left button should be combined with Shift key. Mouse scroll + Shift key can be used for zoom in/out and right click for image movement, or Cmd + left click for Mac users (Figure 3).
Figure 3. Untilted Micrograph Window
Once concluded particle picking process, user can extract particles to be used for reconstruction.