Angular_align_dual_tilt_series

angular_align_dual_tilt_series

Purpose

This program aligns two dual tilt series without the need of any marker. Make sure that the two tilt series have been previously aligned independently and that the tilt axis is vertically set. The program starts aligning the two images at 0º. Then, these alignment parameters are used as starting point for an optimization algorithm that looks for three rotations and three translations relating the two tilt series. The program finally produces a tilt series corresponding to the dual series with the correct Euler angles and shifts so that both tilt series can be reconstructed in a single step.

Usage

$ angular_align_dual_tilt_series ... 

I/O Parameters

• `` Images within the reference tilt series. The header of the images must contain the tilt angle and must be of the form (0, tilt, 0). UseHeader_assign to assign the angles. Images in the selfile must be sorted in ascending order of tilt. The tilt step may vary from one image to the next.
• `` Images within the dual tilt series. The header of the images must contain the tilt angle and must be of the form (0, tilt, 0). UseHeader_assign to assign the angles. Images in the selfile must be sorted in ascending order of tilt. The tilt step may vary from one image to the next.
• `` Rootname for the aligned images. If not given, the dual rootname is used with "_aligned" concatenated.
• -scale [s0.25] of the original size

--Main.CoSS - 04 Jun 2010