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Image_align_tilt_pairs_v31
Center the tilted images of all tilted-untilted image pairs. This program receives as input a metadata with two sets of images, untilted and tilted. Untilted images must have been previously classified and aligned. Then, the tilted images are aligned to their untilted versions. The alignment parameters from the classification plus the alignment parameters from the tilted-untilted comparison are used to deduce the 3D alignment parameters for the tilted images.
Parameters
$: Input metadata with untilted and tilted images $: Output metadata file with rotations & translations for 3D reconstruction. $: 2D average of the untilted images $`--max_shift <value`10> $: Stretch tilted image to fit into the untilted one. Do it only with thin particles $``: Do not align tilted images to untilted ones. Do not align if the quality of the tilted images is low.
To center tilted images allowing a maximum shift of 10 pixels:
xmipp_align_tilt_pairs -i class_00001@classes.xmd -o alignedImages.xmd --max_shift 10