Angular_projection_matching_v31

!xmipp_angular_projection_matching (v3.1)

Usage

Perform a discrete angular assignment using projection matching in real space. This program is relatively fast, using polar coordinates for the in-plane angular searches and the 5-dimensional search of rotation angles and origin offsets is broken in two: first the angles are search in a 3D-search; then, for the optimal orientation the origin offsets are searched (2D).

The output of the program consists of a document file with all assigned angles and rotations. This file also contains a column for the maximum cross-correlation coefficient. Note that the program does not alter the image headers. The recommended use of this program is within the python script of the xmipp_protocol_projmatch.py

See also Angular_discrete_assign_v3 Angular_continuous_assign_v3 Angular_project_library_v3

Parameters

$: Docfile with input images $: Output filename $``: Reference projections $--search5d_shift <s5dshift0> $`--search5d_step &lt;s5dstep`2&gt; $`--Ri <ri`1> $`--Ro <ro`-1> =: Outer radius to limit rotational search ro` -1 -> dim/2-1 -s, --scale <step`1> <n_steps=3>

Extra parameters $--mem <mem1> $--max_shift <max_shift-1> $: CTF to apply to the reference projections, either a CTF parameter file or a 2D image with the CTF amplitudes $`--pad <pad`1> $: Use this if the experimental images have been phase flipped $--thr <threads1> $``: Append (versus overwrite) data to the output file

Examples and notes

Example of use: Sample at 2 pixel step size for 5D shift search

xmipp_angular_projection_matching -i experimental.doc -o assigned_angles.doc --ref reference.stk --search5d_step 2

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