Crystal_angular_projection_matching

crystal_angular_projection_matching

Purpose

This utility allows you to perform a simple (but quick!) 2D-angular assignment using projection matching.

The output of the program consists of a document file with all assigned angles and rotations. This file also contains a column for the maximum cross-correlation coefficient. Upon reading the images, this program applies the translations as already stored in the image headers. This allows iterative use of the program, without the need to assign previously determined alignment parameters.

Although the program is relatively fast, very large datasets may still require significant amounts of CPU.

Usage

$ crystal_angular_projection_matching  ...

Paramters

• `` Selfile with reference images
• `` Selfile with experimenal images
• `` Output file root name
• `` Angular sampling interval (degrees). Small values (e.g.<1 degree) may lead to excessive memory use
• `` If positive: limit search of the 3rd Euler angle to +/-search_psi degrees of the value stored in the image header
• `` If positive: limit search of the 1st Euler angle to +/-search_rot degrees of the value stored in the image header
• `` If positive: limit search of the 2nd Euler angle to +/-search_tilt degrees of the value stored in the image header
• `` Maximum change in origin offsets (+/- pixels)
• `` By default the program modifies the alignment parameters in the headers of the 2D-images, while running. If this flag is set, the image headers are never touched

Examples and notes

A typical use of this program would be:

which would produce the document file with all assigned angles and rotations and modify the headers of all the images in theEXP.sel file.

USER's COMMENTS

--Main.JavierRodriguez - 25 Sep 2007