Angular_assign_continuous_v3

!xmipp_angular_continuous_assign (v3.0)

Usage

Make a continuous angular assignment This program assigns Euler angles to experimental projections by minimizing the difference between the given experimental image and the central slice of a reference volume in Fourier space. All interpolations are based on a B-spline model of the images and the volume. The translations are also optimized. Since an iterative optimization is performed, it must be initialized with some rough estimate of the angles. The output of xmipp_angular_predict can be used as initialization without any transformation. The method is fully described at http://www.ncbi.nlm.nih.gov/pubmed/15885434

Parameters

$``: Input file: metadata, stack, volume or image. Supported read formats are: dm3 : Digital Micrograph 3. img : Imagic. inf,raw : RAW file with header INF file. mrc : CCP4. spe : Princeton Instruments CCD camera. spi, xmp : Spider. tif : TIFF. ser : tecnai imaging and analysis. raw#xDim,yDim,[zDim],offset,datatype,[r] : RAW image file without header file. where datatype can be: uint8,int8,uint16,int16,uint32,int32,long,float,double,cint16,cint32,cfloat,cdouble,bool $--mode <modeoverwrite> where <mode> can be:

• • $-o, --output <output_file> $``: Reference volume $--gaussian_Fourier <s0.5> $`--gaussian_Real &lt;s`0.5&gt; $`--zerofreq_weight <s`0.> $`--max_iter &lt;max`60&gt; $`--max_shift <s`-1> $`--max_angular_change <a`-1>

Examples

A typical use is:

xmipp_angular_continuous_assign -i anglesFromDiscreteAssignment.doc --ref reference.vol -o assigned_angles.txt

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