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IDR_ART
This utility allows you to make reconstructions removing the effect of the CTF. The projection images are modified according to a current guess of the reconstruction and the CTF definition. The modified images should be used for reconstruction which now serves as a new current guess for a new IDR iteration.
Watch out of not having negative values in the CTF since IDR will not converge. Make a phase correction on images first with CorrectPhase.
Input images are rewritten. If you want to preserve them, please, make a copy of them before calling this program.
$ ctf_correct_idr
Parameters
- `` Current guess of the reconstruction
- `` List of images and corresponding CTFs (as ctfparam files)
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-mu [mu1.8] = Value of the accelerating parameter in IDR.
- Make sure that the headers of the projection images are correctly set (i.e., images can be directly used to make a reconstruction)
Here you are an example of CTF corrected: the top left volume is the original phantom, the top right volume is the uncorrected reconstruction, the bottom left is the phase corrected reconstruction, and the bottom right the phase and amplitude corrected one using IDR+ART.
/CTF_correction.gif
An example of call to this program is
$ ctf_correct_idr -vol reconstruction.vol -ctfdat ctfdat.txt
Note that all projection images in ctfdat.txt should be phase corrected and no other transformation should have been applied. Since this program modifies the input images, it is a good advise to make a copy of the phase corrected images before calling the program.
--Main.AlfredoSolano - 17 Jan 2007