SeriesRemoveFluctuations

Series remove fluctuations

Purpose

This program allows you to remove the flickering in a tilt series. This is a phenomenon rather common in X-ray microscopy. For doing so, a low-pass filter of a given frequency (normalized to 0.5) is applied across the time series, i.e., a line is formed with all the lines at the same position along the tilt series. This time series is the filtered and put back into the tilt series.

Usage

$ series_remove_fluctuations ...

Parameter

• `` Input images
• `` Name of the output images. If not given, the input images are rewritten
• -lpf [freq0.25] = Cutoff frequency of the low pass filter

Examples and notes

The most common call is

$ series_remove_fluctuations -i tiltseries.sel

or

$ series_remove_fluctuations -i tiltseries.sel -lpf 0.15

--Main.CoSS - 01 Oct 2009