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Ctf_group_v31

Adrian Quintana edited this page Dec 11, 2017 · 1 revision

!xmipp_ctf_group (v3.1)

Usage

Generate CTF (or defocus) groups from a single CTFdat file. The automated mode groups all images with absolute difference in CTF-values up to a given resolution below a given threshold. For example the example bellow groups all images together with absolute CTF differences smaller than 0.5 up to 15 Angstroms resolution). A complementary manual mode allows to combine different groups or to split) groups up even further.

See also Ctf_create_ctfdat_v3

Parameters

$: Input CTFdat file for all data $`-o <oext`ctf:stk> $`--pad <float`1> $: Output filters for phase-flipped data $: Exclude anisotropic CTFs from groups $: Also calculate Wiener filters $: This sampling rate overwrites the one in the ctf.param files $`--memory <double`1.> $: Compute Groups using 1D CTF, select this option is you have many non astismatic CTFs $--wc <float-1>

MODE 1: AUTOMATED: $--error <float0.5> $--resol <float-1>

MODE 2: MANUAL: $``: 1-column docfile with defocus values where to split the data

Examples and notes

Example of use: Sample using automated mode (resolution = 15 Ang.)

xmipp_ctf_group --ctfdat all_images_new.ctfdat -o[[CtfGroupsNew]]/ctfAuto   --wiener --wc -1 --pad 2 --phase_flipped --error 0.5 --resol 15
Example of use: Sample using manual mode (after manual editing of ctf_group_split.doc)
xmipp_ctf_group --ctfdat all_images_new.ctfdat -o[[CtfGroupsNew]]/ctfManual --wiener --wc -1 --pad 2 --phase_flipped --split[[CtfGroupsNew]]/ctf_group_split.doc

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