PreprocessParticles

!Preprocess Particles

• For each of the selected micrographs, extract the particles as individually windowed images from the micrographs. This option can be performed using either single micrographs or tilt pairs.
• Normalize the images to have zero-mean and a standard deviation of one for the background pixels.
• Create a single selection file containing all particles, sort them based on general statistics to identify outliers.

Example Data

• Input

  • micrograph pair in RAW format plus auxiliary files created in the previous protocol. If you have not executed the previous protocol input data is available in preprocess_particles.tar.gz. The Script with suitable parameters for the test data is xmipp_protocol_preprocess_particles.py.

• Output

  • gallery of selected and normalized images and selection file.

File output_preporcess_particles.tar.gz contains the results of executing thexmipp_protocol_preprocess_particles script.

Pressanalyze results button for visualizing the extracted particles

Gallery of untilted (left) and tilted (right) extracted images.

## USER's COMMENTS

--Main.RobertoMarabini - 09 Oct 2007