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Zeiss Imager Z2
Rob Campbell edited this page May 1, 2024
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| Name on microscope | Filter set # | Excitation (nm) | Beam splitter | Emission (nm) | Example fluorophores for cube |
|---|---|---|---|---|---|
| DAPI | 49 | 365 | 395 | 395-495 | DAPI, Dylight 350, Alexa 350, Hoechst, BFP |
| FITC | 44 | 435-515 | 500 | 480-580 | Alexa 488, FITC, GFP, NeuroTrace 500/525, Syto16 |
| AF 568 | 31 | 535-595 | 585 | 560-680 | Alexa 568, Cy3, tdTomato, DsRed, mCherry, mRFP, TRITC |
| Cy5 | 50 | 610-670 | 660 | 590-670 | Alexa 647/660, Cy5, TO-PRO-3 |
| YFP | 46 | 475-525 | 515 | 505-565 | YFP, Cy2, Alexa 488, FITC, NeuroTrace 500/525, SYBR Green 1 |
| CFP | 47 | 416-456 | 455 | 440-520 | CFP, SYTO 45, SYTOX Blue, |
The Chroma Spectraviewer web applet is useful for checking the spectra of fluorophores not liste
| Magnification | Numerical Aperture | Working Distance (mm) | Field of view (mm) | Resolution (um/pixel) | Links |
|---|---|---|---|---|---|
| 2.5 air | 0.12 | 8.7 | 5.32 x 5.32 | 2.6 | Fluar 2.5x/0.12 |
| 5 air | 0.16 | 12.1 | 2.66 x 2.66 | 1.3 | Plan-Apochromat 5x/0.16 |
| 10 air | 0.45 | 2 | 1.33 x 1.33 | 0.65 | Plan-Apochromat 10x/0.45 Ph1 |
| 20 air | 0.8 | 0.55 | 0.665 x 0.665 | 0.325 | Plan-Apochromat 20x/0.8 |
| 40 air | 0.95 | 0.25 | 0.332 x 0.332 | 0.163 | Plan-Apochromat 40x/0.95 Corr Ph3 |
| 63 oil | 1.4 | 0.19 | 0.211 x 0.211 | 0.103 | Plan-Apochromat 63x/1.40 Oil DIC |