EMBO Practical course CEM3DIP 2020

CryoEM Image Processing Course Material

• Presentation

  • Scipion image processing tutorial

• Import Data

  • Mouse apoferritin
    • Raw data: EMPIAR ID: 10248
    • 3D map: EMD-9865
    • Atom structure reference: PDB-6S61

Model Building Course Material

• Presentation

  • Scipion model building tutorial
  • PPT presentation (model building)
  • Haemoglobin Movie
  • Poster (General model building workflow)

• Import Data

  • BlastP (search for homologues)
  • Human haemoglobin
    • 3D map: emd_3488.map, available directory Data
    • sequence:
      • alpha subunit: UniProt ID P69905 (Homologous sequences: P01958, P01948, P81023, P83134) link
      • beta subunit: UniProt ID P68871 (Homologous sequences: P02062, P02057, G1U9Q8, P83133) link
    • PDB reference: 1pbx (antartic fish: Pagothenia bernacchii) link

• Coot Tutorial

  • 3D map
  • atomic model

• EMDB submission link

  • Haemoglobin in PDB: ID 5ni1
    • Haemoglobin PDB full validation report

• Contacts AD type V (Zhou)

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