AgendaJune2023

Schedule and contents

*First Day:

[ * Extra 90 min: Welcome and presentation of the course, dynamics and mentors, introduction to scipion and aws. (if we are the first ones)]

• 11:30:

  • Introduction to model building in cryo-EM (th-M)
  • How to get a first estimation of the model (homology, alphafold, modelangelo) (th-M)

• 12:15:

  • Presentation of the data used in the practical session
  • Kahoot (R)

• 12:45 min: Map preprocessing (p-R)

• 13:30: lunch

• 14:30: getting a first estimate of the model (th-M)

• 16:00 coffee break

• 16:30: Structure analysis: Structure comparison (M)

• 17:00: Rigid fitting of initial models (M)

• 18:00-30: End of the day

*Secons day:

• 9:30: Review of the previous day and kahoot. (M)
• 10:00: Flexible fitting I (R-coot)
• 11:00: Break
• 11:30: Flexible fitting (continuation) and validation (M-phenix)
• 13:30: lunch
• 14:30: Flexible fitting (continuation) and validation (cont) (M-phenix)
• 16:00: coffee break
• 16:30: Model building of the Beta sub-unit
• 18:00-30: end of the day

*third day:

• 9:30: Review of the previous day and kahoot. (M)
• 10:00: Presentation of the course questionnaire (R)
• 10:15: Building the model of the protein complex (M-join chains)
• 11:00: Coffee Break
• 11:30: Submission of structures (R-EMDB report) *
• 12:00: Structure analysis: Chain contacts (R)
• 13:00: INSTRUCT short message and farewell
• 13:30: lunch and end of the day