july_8_2019

Course Material

• Scipion model building tutorial
• PPT presentation (model building) (marta)
• Hemoglobine Movie
• Coot Tutorial (roberto)
  • 3D map
  • atomic model
• EMDB submission link (marta)
• Hemoglobine
  • 3D map
  • sequence
  • PDB reference

====== 1 Introduction to Model building marta, present model buildiong 2 Problem to solve: Hemoglobin marta, present tutorial data and aim

SCIPION 4 Import Input data roberto 5 3D Map preprocessing (sharpening, unit cell) roberto 6 Structure Prediction by Sequence Homology. Searching for Homologues (web site and chimera) roberto

7 Moving from sequence to atomic structure scenario (marta) 8 Merging 3D Maps and Atomic Structures: Rigid Fitting (marta)

extra emdb validation (marta)

extra tutorial coot (roberto) 9 Refinement: Flexible fitting (roberto), coot, refmac, realspcae refinement en cada paso subir a emdb 10 Structure validation and comparison(roberto)

Subunidad beta (work by yourself) (marta)

11 Building the unit cell (marta) 12 The whole macromolecule (marta) contancts (marta)

encuesta?

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