Comparison_among_predictions

• Here we are going to compare the different types of predictions by AlphaFold, Modelangelo and homology modeling with the submitted structure of the human hemoglobin with PDB ID 5ni1

1. Import the published atomic structure: [5ni1] (https://www.rcsb.org/structure/5ni1) For details look at the Modeling tutorial and the protocol appendix

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2. Comparison among the published structure 5NI1 and AlphaFold, ModelAngelo and the homology modeling predictions: For details look at the appendix

!Compare structures with ChimeraX- operate protocol:

Recommended actions: 1.- Align the AlphaFold prediction to the published structure:

• mmaker #3 to #2

2.- Make movements easier with:

• ~cofr

3.- Select the same part of the structure:

• sel #2/B:320-334

• sel #3/B:320-334

• sel #4/Ae:225-240

4.- At the end save session:

• scipionss

You should see something like this:

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7. Superposition of one structure to other: For details look at the appendix

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7b. Superposition of ModelAngelo prediction to 7F3U structure

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7c. Superposition of AlphaFold prediction to 7F3U structure