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sbx_assembly

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A Sunbeam extension for assembly of contigs using Megahit, gene annotation using Prodigal, and annotation using Blast and Diamond. It can also map reads to contigs and calculat per-base coverage using Minimap2 and samtools.

Configuration

sbx_assembly

  • min_length: Is the minimum contig length to accept in the final filtering abx_annotation
  • threads: Is the number of threads to use running blast/diamond
  • circular_kmin: Is the minimum length of overlap to check for circular contigs with
  • circular_kmax: Is the maximum length of overlap to check for circular contigs with
  • circular_min_len: Is the minimum sequence length to consider checking for circularity sbx_coverage
  • threads: Is the number of threads to use while running minimap2 and samtools sort

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Sunbeam extension for assembly (megahit) and annotation (prodigal)

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