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This repository contains scripts for the Natura2000 reporting of 2025 of invertebrates of Greece as part of ARTICLE 17 of the Directive 92/43/EEC

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Analysis of the invertebrates of Greece

This repository contains the scripts for the analysis and visualisation of the monitoring of invertebrates as part of the Natura2000 Species directive (Art. 17 under the directive 43/92/ECC).

This work is funded by the Natural Environment & Climate Change Agency during the period 10-2024 until 07-2025.

Contents

Scripts

The flowchart of the scripts for the calculation of FRVs The scripts in this repository are (with the order of execution):

  1. species_enrichment.R

This script facilitates species info and occurrences gathering and homogenisation from multiple sources for Greek invertebrates. It starts by importing data from various sources, including GBIF, previous monitoring data (E1X MDPP, E1X DB, E1X reference data), IUCN redlist, NECCA redlist and other sources. To ensure consistency across datasets, the script standardizes column names based on Darwin Core (e.g., renaming decimalLongitude, decimalLatitude) and filters out incomplete records. The main output of the is species_occurrences_art17_invertebrates.tsv file which contains all data from the aformentioned resources. For visualization, the script generates maps for each species, with points colored based on their dataset source.

  1. spatial_analysis.R, spatial data trimming and final enrichment of species occurrences
  2. frvs_invertebrates_gr.R
  3. N2000_targets_invertebrates_gr.R

The script data_validation_qc.R is for the data validation of the data from the current samplings in order to find errors prior to submission.

The script necca_db_standards.R aims to construct all the data compliled from this work in a compatible form with the NECCA database.

Species enrichment

Table with invertebrates information

Name Format Content Source
Species Taxonomy Tabular (xlsx) Species info Manual curation - EIONET
Directive value Tabular (xlsx) Species info ENVENCO
Current value Tabular (xlsx) Species info Παραδοτέο Ανάδοχου
IUCN Red list Tabular (tsv) Species info IUCN
IUCN Red list Tabular (csv) Species occurrences IUCN
NECCA Redlist Tabular (xlsx) Species info NECCA db
NECCA Redlist Tabular (xlsx) Species occurrences NECCA db
Art 17 1.3.1 Tabular (xlsx) Species info EIONET
Natura2000_v32 Access Species info EIONET
Gbif Tabular (tsv) Species occurrences Gbif
ΕΠΟΠΤΕΙΑ Ι Tabular (xlsx) Species occurrences ENVENCO
ΕΠΟΠΤΕΙΑ Ι Tabular (xlsx) Species info ENVENCO
ΟΦΥΠΕΚΑ έργα Μονάδων Tabular (xlsx) Species occurrences NECCA db
Edaphobase 2.0 Tabular (csv) Species occurrences Edaphobase 2.0
National Report 2013-2018 Shp Species info ENVENCO
Molluscabase ? Species occurrences molluscabase.org
Worms Traits Tabular (tsv) Species info
Genetic diversity
Species threats?

Invertebrate species of Annex II of article 17 for the Natura2000

Apatura metis Astacus astacus Austropotamobius torrentium Bolbelasmus unicornis Buprestis splendens Catopta thrips Cerambyx cerdo Coenagrion ornatum Cordulegaster heros Dioszeghyana schmidtii Eriogaster catax Euphydryas aurinia Euplagia quadripunctaria Hyles hippophaes Lindenia tetraphylla Lucanus cervus Lycaena dispar Maculinea arion Ophiogomphus cecilia Papilio alexanor Paracaloptenus caloptenoides Parnassius apollo Parnassius mnemosyne Polyommatus eroides Probaticus subrugosus Proserpinus proserpina Pseudophilotes bavius Rhysodes sulcatus Rosalia alpina Stenobothrus eurasius Stylurus flavipes Unio crassus Unio elongatulus Vertigo angustior Vertigo moulinsiana Zerynthia polyxena Morimus asper funereus Osmoderma eremita Complex Hirudo verbana

Endemic species and critically endangered are also studied in order to include them in future analyses.

IUCN Redlist

Search in the website for the following: Taxonomy: Annelida - PhylumRemoveArthropoda - PhylumRemoveCnidaria - PhylumRemoveEchinodermata - PhylumRemoveMollusca - PhylumRemovePorifera - PhylumRemove Land Regions: Greece Include: Species

Didn't have any points data for the invertrebrate species of art 17.

Another search with redlist_species_data_f4d05b4d-bdbd-4b10-8c67-1523dffd9d0e id.

Geographical Scope: Global Land Regions: Greece Include: Species

All the data available from iunc Search on 2024-10-30 at 08:47:29

gawk -F"," '(NR>1){a[$3]++}END{for (i in a){print i "\t" a[i]}}' points_data.csv | sort -n -k 3

614 species with points data with 753110 occurrences.

This database doesn't have any occurrences of the species of interest.

GBIF database

Here we retrieve all the occurrences of the species from GBIF.

Edaphobase 2.0

The download is manual with a registered account. Once the download is finished the user can filter based on species or country or some other field. Here we first filtered with country code of Greece to create a new file to further analyse it in R script.

gawk -F";" '(NR==1 || $7~"Greece"){print $0}' 2025-02-26-edaphobase-export.csv > 2025-02-26-edaphobase-export_GR.csv

This database doesn't have any occurrences of the species of interest.

Spatial analysis

Table with spatial data

Name Format Content Source
Natura 2000 Habitat types shp habitat Κτηματολόγιο (ΕΚΧΑ)
Natura 2000 vegetation types shp vegetation Διεύθυνση δασών
Corine Land Cover shp Land use EEA
Natura2000 shp Natura2000 polygons EIONET
EAA grid shp 1km², 10km² EEA
Digital Elevation model tiff EEA
Slope
Exposure
Springs ? ?
WorldClim tiff WorldClim 2.0
GlobCover ? ?
HildaPlus tiff Land cover change from 1960
Gadm shp Administrative units Gadm
Υδρογραφικό δίκτυο Ελλάδα shp ?
Natura2000 threats ?
Elstat

Natura2000

The MS access database has the schema of EEA Natura2000 in access format.

To retrieve each table run the following

mdb-export -d "\t" Natura2000DB_V32.mdb other_species > Natura2000DB_V32_other_species.tsv

The EEA Natura2000 data.

DEM

  • digital elevation model over Europe (EU-DEM) eurostat DEM
  • slope, aspect

Slope

Favourable Reference Values

The paper: Defining applying the concept of Favourable Reference Values for species and habitats defines the FRVs.

MaxEnt

The following variables are used mostly:

  • Altitude
  • Slope
  • Aspect
  • Annual Mean Temperature
  • Temperature Seasonality
  • Mean Temp of Wettest Quarter
  • Annual Precipitation
  • Precipitation Seasonality
  • Precipitation of Warmest Quarter

Natura2000 targets

Data validation and quality control

NECCA database

It is under development from AUTH. There is a script under development for the standardisation of data in order to be uploaded to NECCA database.

Software

R version 4.4.3 (2025-02-28) with the packages:

  • tidyr_1.3.1
  • tibble_3.2.1
  • ggplot2_3.5.1
  • readr_2.1.5
  • purrr_1.0.2
  • dplyr_1.1.4
  • readxl_1.4.3
  • rgbif_3.8.1
  • vegan_2.6-10
  • taxize_0.10.0
  • terra_1.8-15
  • sf_1.0-19

GNU Awk 5.3.1

Licence

GNU GPLv3 license (for 3rd party scripts separate licenses apply).

About

This repository contains scripts for the Natura2000 reporting of 2025 of invertebrates of Greece as part of ARTICLE 17 of the Directive 92/43/EEC

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