docker pull poulphunter/openrr:latest
To use mafft from NCBI_sequences.fasta
to NCBI_sequences_aligned.fasta
With this example fasta file is in a mounted directory from host: /home/db/
source and destination is mounted in the same directory to avoid confusion.
docker run --mount type=bind,source=/home/db/,target=/home/db/ -i -t poulphunter/openrr:latest mafft --auto --thread 8 -s /home/db/NCBI_sequences.fasta > /home/db/NCBI_sequences_aligned.fasta
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fastqc - Quality check
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trimmomatic - Trimming and Adapter Removal
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samtools - Pre and post processing
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hisat2 - Alignment
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bedtools - Consensus Genome and Variant calling
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bcftools - Consensus Genome and Variant calling
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Varscan - Consensus Genome and Variant calling
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seqtk - Consensus Genome and Variant calling
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Quast - Assembly evaluation and Statistics
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SPAdes - de novo Genome assembly
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MEGAHIT - de novo Genome assembly
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Krona - Evaluation of species diversity and Visualization
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Kraken2 - Evaluation of species diversity and Visualization
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MegaX-CC - Phylogenetic analysis
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mafft - Phylogenetic analysis
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fasttree - Phylogenetic analysis
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FigTree - Phylogenetic analysis
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BMGE ( Block Mapping and Gathering with Entropy) - Phylogenetic analysis
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system utils like : time htop screen nano curl git
docker build -t poulphunter/openrr .