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Adding updates from previous version
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Metagenomics/Illumina/Pipeline_GL-DPPD-7107_Versions/GL-DPPD-7107-A.md

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@@ -20,6 +20,21 @@ Lauren Sanders (OSDR Project Scientist)
2020

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## Updates from previous version
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- The following tool versions were updated:
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- FastQC
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- MultiQC
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- bowtie2
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- samtools
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- CAT
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- gtdbtk
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- HUMAnN3
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- MetaPhIAn3
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- In [step 14d](#14d-mag-taxonomic-classification), MAG taxonomic classification, gtdbtk classify_wf now takes a new argument "--skip_ani_screen" which specifies to skip the ani_screening step to classify genomes using mash and skani.
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# Table of contents
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- [**Software used**](#software-used)
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* `classify_wf` – specifies the workflow being utilized
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* `--genome_dir` – specifies the directory holding the MAGs generated in step 14c
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* `-x` – specifies the extension that is on the MAG fasta files that are being taxonomically classified
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* `-out_dir` – specifies the output directory
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* `-skip_ani_screen` - specifies to skip ani_screening step to classify genomes using mash and skani
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* `--out_dir` – specifies the output directory
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* `--skip_ani_screen` - specifies to skip ani_screening step to classify genomes using mash and skani
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**Input data:**
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