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- Workflow now produces a file called meta.sh (in the 'GeneLab' sub-directory) that contains information about the workflow run. This file is used by the post processing workflow to generate a protocol description. (5a8a255)
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- POST_PROCESSING will now generate a protocol description using the contents of meta.sh and text templates. (801e2ad)
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- Workflow can now be run using an ISA archive by supplying parameter: 'isaArchivePath' (as either a local path or public web uri) (8822069)
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### Changed
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- Update dp_tools from 1.3.2 to 1.3.4 (158ce5e)
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- This updates the POST_PROCESSING workflow assay table to join multiple files by ',' instead of ',<SPACE>' and enables max flag code setting.
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- Slightly reduced stringency in V&V check for log2fc computation to account for rounding errors, specifically from 99.9% of rows within tolerance to 99.5%. (9fd2c11)
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- Publish directory behavior reworked to use the OSD accession as part of the default name. Now uses `resultsDir` instead of `outputDir` as the parameter name when a user does control the published files directory. (97cba72)
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### Fixed
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- Halt level flags now properly trigger workflow halt. (0885175)
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- Boxplots now show all y-axis labels when working with many samples. (7ec10d4s)
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- Density plot legend cex (character expansion) now has a minimum of 0.35 (rather than raising an exception for very large numbers of samples) (9a54fdc)
Copy file name to clipboardExpand all lines: Microarray/Affymetrix/Workflow_Documentation/NF_MAAffymetrix/README.md
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@@ -97,9 +97,9 @@ All files required for utilizing the NF_MAAffymetrix GeneLab workflow for proces
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copy of latest NF_MAAffymetrix version on to your system, the code can be downloaded as a zip file from the release page then unzipped after downloading by running the following commands:
While in the location containing the `NF_MAAffymetrix_1.0.1` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_MAAffymetrix workflow:
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While in the location containing the `NF_MAAffymetrix_1.0.2` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_MAAffymetrix workflow:
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> Note: Nextflow commands use both single hyphen arguments (e.g. -help) that denote general nextflow arguments and double hyphen arguments (e.g. --ensemblVersion) that denote workflow specific parameters. Take care to use the proper number of hyphens for each argument.
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<br>
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#### 3a. Approach 1: Run the workflow on a GeneLab Affymetrix Microarray dataset
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```bash
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nextflow run NF_MAAffymetrix_1.0.1/main.nf \
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nextflow run NF_MAAffymetrix_1.0.2/main.nf \
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-profile singularity \
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--osdAccession OSD-266 \
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--gldsAccession GLDS-266
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> Note: Specifications for creating a runsheet manually are described [here](examples/runsheet/README.md).
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```bash
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nextflow run NF_MAAffymetrix_1.0.1/main.nf \
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nextflow run NF_MAAffymetrix_1.0.2/main.nf \
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-profile singularity \
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--runsheetPath </path/to/runsheet>
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```
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> Note: Specifications for the ISA Tab Archive format can be found [here](https://isa-specs.readthedocs.io/en/latest/isatab.html).
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```bash
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nextflow run NF_MAAffymetrix_1.0.1/main.nf \
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nextflow run NF_MAAffymetrix_1.0.2/main.nf \
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-profile singularity \
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--isaArchivePath </path/to/isaArchive>
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```
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**Required Parameters For All Approaches:**
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* `NF_MAAffymetrix_1.0.1/main.nf` - Instructs Nextflow to run the NF_MAAffymetrix workflow
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* `NF_MAAffymetrix_1.0.2/main.nf` - Instructs Nextflow to run the NF_MAAffymetrix workflow
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* `-profile` - Specifies the configuration profile(s) to load, `singularity` instructs Nextflow to setup and use singularity for all software called in the workflow
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All parameters listed above and additional optional arguments for the NF_MAAffymetrix workflow, including debug related options that may not be immediately useful for most users, can be viewed by running the following command:
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```bash
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nextflow run NF_MAAffymetrix_1.0.1/main.nf --help
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nextflow run NF_MAAffymetrix_1.0.2/main.nf --help
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```
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See `nextflow run -h` and [Nextflow's CLI run command documentation](https://nextflow.io/docs/latest/cli.html#run) for more options and details common to all nextflow workflows.
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All R code steps and output are rendered within a Quarto document yielding the following:
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- Output:
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- NF_MAAffymetrix_1.0.1.html (html report containing executed code and output including QA plots)
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- NF_MAAffymetrix_1.0.2.html (html report containing executed code and output including QA plots)
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The outputs from the Analysis Staging and V&V Pipeline Subworkflows are described below:
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