Repository for data, information, and analyses related to SSWD work from Summer 2022 - looking at immune response in two size classes of Pycnopodia helianthoides exposed to wasting disease.
RNAseq data is from coelomocytes collected between 2022-06-21 (Day 0, pre-treatment) and 2022-07-06 (1-15 days post-treatment). These samples came from our first experiment of the summer.
Does raw tissue inoculum from a wasting Pycnopodia helianthoides cause disaese in small (juvenile) and large (adult) Pycnopodia helianthoides?
Collection information:
Large Pycnpoodia helianthoides:
Collected by WDFW dive team at Dallas Bank, 2022-05-27.
Small Pycnopodia helianthoides:
Collected by WDFW dive team at Langley Dock, 2022-06-03.
Pre-experiment:
Stars were held in isolation and quarantined for 14 days to monitor for signs of wasting. Only healthy-appearing stars were used in the experiment post-quarantine and isolation.
Groups:
Large P. helianthoides (n) = 30
Small P. helianthoides (n) = 28
Large diameter --> Small diameter -->
Large exposed (raw tissue homogenate) = 10
Large exposed (0.22um filtered raw tissue homogenate) = 5
Large control (heat-killed tissue homogenate) = 15
Small exposed (raw tissue homogenate) = 9
Small exposed (0.22um filtered raw tissue homogenate) = 5
Small control (heat-killed tissue homogenate) = 14