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SeRPeNT analysis

This README is intended to provide the necessary steps to reproduce the results, files and figures included in the following publication:

The discovery potential of RNA processing profiles

Amadís Pagès, Ivan Dotu, Joan Pallarés-Albanell, Eulàlia Martí, Roderic Guigó and Eduardo Eyras

bioRxiv

Getting started

Prerequisites

Git - http://git-scm.com/

Perl - https://www.perl.org/get.html

Python - https://www.python.org/downloads/

R - https://www.r-project.org/

BEDTools - https://github.com/arq5x/bedtools2/releases/latest

SRA Toolkit - http://www.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software

SeRPeNT - https://bitbucket.org/regulatorygenomicsupf/serpent/downloads

STAR - https://github.com/alexdobin/STAR/releases

Infernal - http://eddylab.org/software/infernal/

cutadapt - https://github.com/marcelm/cutadapt

Clone serpent-analysis

git clone https://amadis_pages@bitbucket.org/regulatorygenomicsupf/serpent-analysis.git
cd serpent-analysis

Edit software dependencies

Edit mks/mkdefs.makefile to set up software dependencies

Running the analyses

Benchmarking SeRPeNT

make benchmarking

Generating the extended annotation

make annotation

Running the differential processing analysis

make diffproc

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