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ceugenia/README.md

👋 Hi there, my name is Constanza Perez! (AKA ceugenia)

Microbiologist🪴Computational Biologist bridging AI & biology | Rare-cell detection, scRNA-seq, and interactive genomics tools 🚀👾

I’m a researcher at UC Riverside’s MIGCrest Lab, passionate about leveraging machine learning and bioinformatics to decode complex biological systems. My current work focuses on single-cell transcriptomics, neural crest development, and building reproducible tools that make genomic data more accessible and interpretable.


🖥️ Current Focus

  • Rare-cell population detection in single-cell RNA-seq (scRNA-seq) using CIARA and BigSur
  • Building interactive Shiny apps for exploring gene expression in early development
  • Developing reproducible workflows in R and Python (Seurat, Scanpy, Snakemake, Nextflow)
  • Researching neural crest lineage segregation during early embryogenesis

🛠️ Tech Stack

Languages & Environments:
Python R Bash SQL Jupyter Colab

Bioinformatics & ML:
Scikit-learn Pandas NumPy Seurat Scanpy XGBoost

Visualization & Tools:
Shiny ggplot2 Plotly Git Docker


📁 Featured Projects

R pipeline for identifying rare cell populations (neural crest stem cells, immune subsets) using BigSur.
Outcome: End-to-end reproducible workflow with Conda environment management and publication-ready visuals.
Tech: R, BigSur, Seurat, Conda, UMAP, QC plots


Automated quantification of PTBP1 (RRM2 domain) localization from heterokaryon assays using ImageJ/Fiji and Python.
Outcome: Analyzed 26,040 cells; identified novel nuclear retention pattern; presented initial results for this project at CSUF Research Symposium (2021).
Tech: ImageJ, Python, OpenCV, scikit-image, Dash


🔬 CIARA-MGCrestLab-SC 🔒 (Private)

Benchmarking CIARA vs BigSur on human gastrula datasets (GSE136447, E-MTAB-9388).
Outcome: CIARA achieved recall = 0.92 at low read depth; identified rare hemogenic endothelial progenitors.
Tech: R, Seurat, Python, Jupyter, UMAP, Volcano Plots


🧪 MIGCrestLab ShinyApp 🔒 (Private)

Interactive Shiny app for exploring Seurat clusters, gene expression, and marker heatmaps in neural crest datasets.
Outcome: Reduced data exploration time by ~40% for wet-lab collaborators.
Tech: R, Shiny, Seurat, ggplot2, Plotly, VennDiagram


👩‍💻 Experience

Assistant Researcher @ MIGCrest Lab, UC Riverside

(2023 – Present)

  • Built scRNA-seq pipelines (Seurat, CIARA, BigSur) for rare population detection
  • Developed a ShinyApp used by the lab for exploratory data analysis
  • Integrated multi-omics data (Visium spatial transcriptomics) with transcriptomic results

(May – Jun 2024)

  • Applied ML models (XGBoost, Random Forest, Logistic Regression) to clinical + omics datasets
  • Delivered reproducible workflows in Python with scikit-learn

📚 Education

  • B.S. Microbiology @ University of California, Riverside (2023 – 2025)
  • Machine Learning for Medicine Track @ SCIP - Science Coding Immersion Program (2024)
  • Biotechnology Laboratory Assistant Certificate @ Fullerton College (2020)

🪴 Interests

  • Open science and reproducible workflows
  • Single-cell + spatial multi-omics integration
  • Rare-cell algorithm development
  • Ethical AI in biomedical research
  • Mentoring students in computational biology

📬 Let’s Connect

LinkedIn
Email

👾 Explore my repositories below!


Pinned Loading

  1. immune-chord immune-chord Public

    An end-to-end R pipeline utilizing the BigSur algorithm for robust detection and analysis of rare cell populations in single-cell RNA sequencing data.

    R 2

  2. ptbp1-imagej-analysis ptbp1-imagej-analysis Public

    Automated ImageJ/Fiji and Python pipeline for quantifying PTBP1 and RRM2 domain subcellular localization from fluorescence microscopy images in heterokaryon assays.

    Jupyter Notebook 2