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Phosphoproteomics of ERK1/2 regulation of neural differentiation (second analysis)

Software used for phosphoproteomics analysis in the manuscript (soon to be published).

Maintainer: Marek Gierlinski

Collaborators: Elisenda Raga Gil, Kate Storey

Usage

The software in this repository is build as a targets pipeline in R. We recommend using Positron or RStudio for running the pipeline.

Open an R console in Positron or RStudio and create the environment using renv:

install.packages("renv")
renv::restore()

This will install all the required packages. If anything is missing, install additional packages locally using renv::install(). You can check if all packages are installed by running source("_targets.R"). If no errors are produced, you are good to go.

Once the environment is set up, run the targets pipeline:

targets::tar_make()

This will perform all calculations, generate data objects, figures, and tables (as targets objects), and output TSV files in the ./tab directory. An HTML report will be generated in the ./doc directory.

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Re-analysis of phosphorylation data, including total proteome (Storey)

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