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Barcoding for Beginners is a project funded by a Royal Society Partnership Grant. It enables students to engage in real scientific research using molecular biology and bioinformatics techniques, while gaining practical skills and exploring careers in genomics.
The program consists of 10 one-hour sessions, and begins with educators participating in hands-on professional development. Afterwards, schools receive equipment loans, consumables, teaching resources, and support from genomics professionals, enabling them to extend the experience to students.
Participants will:
- Collect invertebrate samples
- Extract DNA
- Amplify a DNA barcode using PCR
- Visualize DNA using gel electrophoresis
- Sequence DNA and identify samples through bioinformatics
- Present their findings via scientific posters
Aim: Understand the barcoding process, bioinformatics basics, and plan for sample collection.
Includes:
- Educator notes
- Student notes- Barcoding Basics
- PowerPoint presentation
- BLAST results table
- Declaration of consent form
- Sample information record sheet
- Unknown barcodes sheet
- 02_Bioinformatics and BLAST instructions
Aim: Interpret chromatograms and use bioinformatics tools to identify invertebrate samples.
Includes:
Aim: Create a phylogenetic tree to explore evolutionary relationships using multiple sequence alignment.
Includes:
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