Amplicon-Separator

A pipline for amplicons from different population separation (Suitable for illumina sequencing platform)

step1:

• Dependencies:
  pandaseq 2.11

Using Overlapper.py for overlapping splicing
Please input pair-end sequencing result fastq1,fastq2 and the path of <output.fa>

step2:

• Dependencies:
  seqtk 1.3
  fastx_toolkit 0.0.14

Using Separator.bash for amplicons separating
Please input <output.fa from step1> and primers file
The formate of primers file was show in Merge_DNA_primer.txt