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Wrapper around the dada2 workflow to process 16S rRNA gene sequencing data at RIVM-IIV

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RIVM-IIV-Microbiome/modularDADA2

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ModularDADA2

Converting the dada2 big data workflow into a modular pipeline for analysing multiple sequencing libraries.

Divided into

  • Preprocessing
  • Infer Sequence Variants and remove chimera
  • Taxonomic assignments

Codes

This is accomplished by set of three functions.

  • Preprocessing

    • filterTrimReads (R/01_preprocessing.R)
  • Infer Sequence Variants and remove chimera

    • inferSequenceVariantsBD (R/02_inferSequenceVariantsBD.R)
  • Taxonomic assignments

    • classifyASV (R/03_taxonomyAssignments.R)

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Wrapper around the dada2 workflow to process 16S rRNA gene sequencing data at RIVM-IIV

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LICENSE.md

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