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Abstraction and object orientation of the project structure has been improved: Reusable functions were restructured as extendable classes; only locally used functionality was outsourced to internal classes; redundant or unusable code was deleted.
Additional runtime and memory optimization: In particular, VCF processing and sequence export have been improved through capacity estimation and improved algorithms.
Full review of the code documentation: JavaDoc is now hosted on a GitHub Pages website.
Update of SnpEff to the latest version (5.2f 2025-02-07).
Improved CLI logging and user information as well as input parameter processing and description.
New functions added: Feature validation compliant to the SO. Reference-free processing (without any reference sequence).