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A Python tool for designing primers from DNA sequences in FASTA format. Supports multiple sequences at once. Uses Biopython and Primer3 to generate primers, calculate GC content, melting temperature (Tm), and predicted product size.

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HMShahzaib/primer-design-pipeline

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primer-design-pipeline

A Python tool for designing primers from DNA sequences in FASTA format.
Supports multiple sequences at once.
Uses Biopython and Primer3 to generate primers, calculate GC content, melting temperature (Tm), and predicted product size.


Usage

Run the script:

python primer_design.py

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A Python tool for designing primers from DNA sequences in FASTA format. Supports multiple sequences at once. Uses Biopython and Primer3 to generate primers, calculate GC content, melting temperature (Tm), and predicted product size.

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