Skip to content

should we define "ligation_site_regex" if we choose enzyme "none"? #179

@shengqh

Description

@shengqh

Currently, we set enzyme "none" and didn't set ligation_site_regex in configuration json file since we used MNase. Based on the wdl code, the ligation_site would be determined as "XXXX" in hic-pipeline. However, in call-calculate_stats_on_library task, the stats_0_lib0.txt looks weird. Should we set ligation_site_regex to something else even the enzyme is none?

Read type: Paired End
Sequenced Read Pairs: 4144224
No chimera found: 187008411 (4512.51%)
One or both reads unmapped: 187008411 (4512.51%)
2 alignments: 72160909 (1741.24%)
2 alignments (A...B): 43056597 (1038.95%)
2 alignments (A1...A2B; A1B2...B1A2): 29104312 (702.29%)
3 or more alignments: 0 (0.00%)
Ligation Motif Present: N/A
Average insert size: 0.00
Total Unique: -68843076 (-95.40%, -1661.18%)
Total Duplicates: 141003985 (195.40%, 3402.42%)

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions