Hi,
I am attempting to run lefse using the command "run_lefse", to analyse for differential microbes across 2 clusters that are significant by PERMANOVA.
However, it is the first time I am encoutering an error:
Error in purrr::map2_lgl():
! Can't recycle .x (size 45) to match .y (size 0).
This is my code:
lefse_results <- run_lefse(ps_tmp, group = "labels", # labels are my groups of interest
taxa_rank = "none",
norm = "CPM",
kw_cutoff = 0.05,
lda_cutoff = 2,
wilcoxon_cutoff = 0.05,
multigrp_strat = FALSE,
strict = "0" )
Below is some information of my phyloseq object, if that helps:
phyloseq-class experiment-level object
otu_table() OTU Table: [ 24 taxa and 45 samples ]
sample_data() Sample Data: [ 45 samples by 349 sample variables ]
tax_table() Taxonomy Table: [ 24 taxa by 6 taxonomic ranks ]
Thank you and hoping to get some kind help!
Kai Xian