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Description
Bruno is using the following scripts to load the sweet cherry genome and gene annotations. These scripts are more automated and compliant with EBI metadata. To be consistent with Epl, Gramene may want to adopt the same process. The scripts he used are
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Genome Loader
https://github.com/Ensembl/plant_tools/blob/master/core/load_genome_shell.pl
This script directly import genome assembly and meta data from ENA database and load into ensembl core database, I assume it takes care of the global variables in ensembl_production. -
GFF loader
https://github.com/Ensembl/plant_tools/blob/master/core/load_GFF_hive.pl
He had to fix the GFF file (contig names, pseudogenes, RecName descriptions, no mRNA features) before use this script, this script will initiate an ehive pipeline to do the loading, it was recommended to run on a execution nodes not a login node. -
Run the core analyses
He then annotate repeats, xrefs, metadata and run the healthchecks,