exit code -6 #373
Description
i have around 60 samples, that performed paired-end seq for metagenome, total ~400G clean data.
i have combine all forward reads and reverse reads, gained F.fastq,gz and R.fastq.gz. and than i run:
megahit -1 /home/dell/raw_data/F.fastq.gz -2 /home/dell/raw_data/R.fastq.gz -o megahit_out --out-prefix assembly -m 0.5 -t 25
94.313Gb memory in total.
Using: 47.157Gb.
MEGAHIT v1.1.3
--- [Thu Jun 27 18:05:02 2024] Start assembly. Number of CPU threads 25 ---
--- [Thu Jun 27 18:05:02 2024] Available memory: 101267902464, used: 50633951232
--- [Thu Jun 27 18:05:02 2024] Converting reads to binaries ---
b' [read_lib_functions-inl.h : 209] Lib 0 (/home/dell/raw_data/F.fastq.gz,/home/dell/raw_data/R.fastq.gz): pe, 5694767552 reads, 150 max length'
b' [utils.h : 126] Real: 9029.7895\tuser: 4891.5591\tsys: 609.5392\tmaxrss: 150828'
--- [Thu Jun 27 20:35:32 2024] k list: 21,29,39,59,79,99,119,141 ---
--- [Thu Jun 27 20:35:32 2024] Extracting solid (k+1)-mers for k = 21 ---
Error occurs when running "sdbg_builder count/read2sdbg", please refer to megahit_out/assembly.log for detail
[Exit code -6]
my computer memory is 96G, and ~1T space available.
So I want to know the specific reason for the termination, and if there is any good analysis process recommended based on the existing computer?
Looking forward to your reply very much
Thanks