Refer to the Nextflow documentation for installation instructions.
First, build the singularity image using the provided definition file:
singularity build --fakeroot aiap-nf.sif aiap-nf.def
Alternatively, you can use any other singularity image that contains the required tools.
- Sample Sheet: Edit
samplesheet-demo.csv(filename can be changed) to include your samples. - Configuration: Edit
params-demo.json(filename can be changed) to specify your pipeline parameters.
You can run the pipeline using:
nextflow run tyfu-cc/aiap -params-file /path/to/params/json/file -with-singularity /path/to/your/sif/file
Or, if you've cloned the pipeline locally, navigate to its directory and run:
nextflow run main.nf -params-file /path/to/your/params/json/file -with-singularity /path/to/your/sif/file