|
3223 | 3223 | "id": "e7b77012", |
3224 | 3224 | "metadata": {}, |
3225 | 3225 | "source": [ |
3226 | | - "## Quiz\n", |
3227 | | - "\n", |
3228 | | - "Where does the high variability of AIRs stem from?\n", |
3229 | | - "- \\+ Junctional diversity adds random nucleotides.\n", |
3230 | | - "- Peripheral diversity motivates rapid amino acid switches specific to the host organ.\n", |
3231 | | - "- \\+ Combinatorial diversity of different gene segments.\n", |
3232 | | - "- Inherited diversity genetically encodes multiple AIRs.\n", |
3233 | | - "\n", |
3234 | | - "What properties does a productive AIR have?\n", |
3235 | | - "- \\+ It spans over the V- to the J-gene with an CDR3 regions contained.\n", |
3236 | | - "- It contains a stop codon in the D-gene.\n", |
3237 | | - "- There exist multiple different sequences for the same AIR chains.\n", |
3238 | | - "- \\+ It contains a start codon in the leading region.\n", |
| 3226 | + "## Quiz" |
| 3227 | + ] |
| 3228 | + }, |
| 3229 | + { |
| 3230 | + "cell_type": "code", |
| 3231 | + "execution_count": 2, |
| 3232 | + "id": "35d2d491", |
| 3233 | + "metadata": { |
| 3234 | + "tags": [ |
| 3235 | + "remove_input" |
| 3236 | + ] |
| 3237 | + }, |
| 3238 | + "outputs": [ |
| 3239 | + { |
| 3240 | + "data": { |
| 3241 | + "text/html": [ |
| 3242 | + "\n", |
| 3243 | + " <style>\n", |
| 3244 | + " div#flip-card .output.text_html {\n", |
| 3245 | + " background-color: transparent;\n", |
| 3246 | + " }\n", |
| 3247 | + " .flip-card-q1 {\n", |
| 3248 | + " background-color: transparent;\n", |
| 3249 | + " width: 350px;\n", |
| 3250 | + " height: 200px;\n", |
| 3251 | + " perspective: 1000px;\n", |
| 3252 | + " display: inline-block;\n", |
| 3253 | + " margin: 10px;\n", |
| 3254 | + " text-align: center;\n", |
| 3255 | + " padding: 10px;\n", |
| 3256 | + " }\n", |
| 3257 | + " .flip-card-inner-q1 {\n", |
| 3258 | + " position: relative;\n", |
| 3259 | + " width: 100%;\n", |
| 3260 | + " height: 100%;\n", |
| 3261 | + " text-align: center;\n", |
| 3262 | + " transition: transform 0.6s;\n", |
| 3263 | + " transform-style: preserve-3d;\n", |
| 3264 | + " transform-origin: center;\n", |
| 3265 | + " }\n", |
| 3266 | + " .flip-card-q1:hover .flip-card-inner-q1 {\n", |
| 3267 | + " transform: rotateY(180deg);\n", |
| 3268 | + " }\n", |
| 3269 | + " .flip-card-front-q1, .flip-card-back-q1 {\n", |
| 3270 | + " position: absolute;\n", |
| 3271 | + " width: 100%;\n", |
| 3272 | + " height: 100%;\n", |
| 3273 | + " min-height: 200px;\n", |
| 3274 | + " backface-visibility: hidden;\n", |
| 3275 | + " display: flex;\n", |
| 3276 | + " align-items: center;\n", |
| 3277 | + " justify-content: center;\n", |
| 3278 | + " font-weight: bold;\n", |
| 3279 | + " color: white;\n", |
| 3280 | + " padding: 20px;\n", |
| 3281 | + " border-radius: 15px;\n", |
| 3282 | + " box-sizing: border-box;\n", |
| 3283 | + " overflow: hidden;\n", |
| 3284 | + " text-align: center;\n", |
| 3285 | + " }\n", |
| 3286 | + " .flip-card-front-q1 {\n", |
| 3287 | + " background-color: #3965a3;\n", |
| 3288 | + " font-size: 20px;\n", |
| 3289 | + " }\n", |
| 3290 | + " .flip-card-back-q1 {\n", |
| 3291 | + " background-color: #a8d480;\n", |
| 3292 | + " transform: rotateY(180deg);\n", |
| 3293 | + " font-size: 13px;\n", |
| 3294 | + " }\n", |
| 3295 | + " </style>\n", |
| 3296 | + "\n", |
| 3297 | + " <div class=\"flip-card-q1\">\n", |
| 3298 | + " <div class=\"flip-card-inner-q1\">\n", |
| 3299 | + " <div class=\"flip-card-front-q1\">\n", |
| 3300 | + " Where does the high variability of AIRs stem from?\n", |
| 3301 | + " </div>\n", |
| 3302 | + " <div class=\"flip-card-back-q1\">\n", |
| 3303 | + " Junctional diversity adds random nucleotides. Peripheral diversity motivates rapid amino acid switches specific to the host organ. Combinatorial diversity of different gene segments. Inherited diversity genetically encodes multiple AIRs.\n", |
| 3304 | + " </div>\n", |
| 3305 | + " </div>\n", |
| 3306 | + " </div>\n", |
| 3307 | + " <noscript>\n", |
| 3308 | + " <p><strong>Q:</strong> Where does the high variability of AIRs stem from?</p>\n", |
| 3309 | + " <p><strong>Answer:</strong> Junctional diversity adds random nucleotides. Peripheral diversity motivates rapid amino acid switches specific to the host organ. Combinatorial diversity of different gene segments. Inherited diversity genetically encodes multiple AIRs.</p>\n", |
| 3310 | + " </noscript>\n", |
| 3311 | + " " |
| 3312 | + ], |
| 3313 | + "text/plain": [ |
| 3314 | + "<IPython.core.display.HTML object>" |
| 3315 | + ] |
| 3316 | + }, |
| 3317 | + "metadata": {}, |
| 3318 | + "output_type": "display_data" |
| 3319 | + }, |
| 3320 | + { |
| 3321 | + "data": { |
| 3322 | + "text/html": [ |
| 3323 | + "\n", |
| 3324 | + " <style>\n", |
| 3325 | + " div#flip-card .output.text_html {\n", |
| 3326 | + " background-color: transparent;\n", |
| 3327 | + " }\n", |
| 3328 | + " .flip-card-q2 {\n", |
| 3329 | + " background-color: transparent;\n", |
| 3330 | + " width: 350px;\n", |
| 3331 | + " height: 200px;\n", |
| 3332 | + " perspective: 1000px;\n", |
| 3333 | + " display: inline-block;\n", |
| 3334 | + " margin: 10px;\n", |
| 3335 | + " text-align: center;\n", |
| 3336 | + " padding: 10px;\n", |
| 3337 | + " }\n", |
| 3338 | + " .flip-card-inner-q2 {\n", |
| 3339 | + " position: relative;\n", |
| 3340 | + " width: 100%;\n", |
| 3341 | + " height: 100%;\n", |
| 3342 | + " text-align: center;\n", |
| 3343 | + " transition: transform 0.6s;\n", |
| 3344 | + " transform-style: preserve-3d;\n", |
| 3345 | + " transform-origin: center;\n", |
| 3346 | + " }\n", |
| 3347 | + " .flip-card-q2:hover .flip-card-inner-q2 {\n", |
| 3348 | + " transform: rotateY(180deg);\n", |
| 3349 | + " }\n", |
| 3350 | + " .flip-card-front-q2, .flip-card-back-q2 {\n", |
| 3351 | + " position: absolute;\n", |
| 3352 | + " width: 100%;\n", |
| 3353 | + " height: 100%;\n", |
| 3354 | + " min-height: 200px;\n", |
| 3355 | + " backface-visibility: hidden;\n", |
| 3356 | + " display: flex;\n", |
| 3357 | + " align-items: center;\n", |
| 3358 | + " justify-content: center;\n", |
| 3359 | + " font-weight: bold;\n", |
| 3360 | + " color: white;\n", |
| 3361 | + " padding: 20px;\n", |
| 3362 | + " border-radius: 15px;\n", |
| 3363 | + " box-sizing: border-box;\n", |
| 3364 | + " overflow: hidden;\n", |
| 3365 | + " text-align: center;\n", |
| 3366 | + " }\n", |
| 3367 | + " .flip-card-front-q2 {\n", |
| 3368 | + " background-color: #3965a3;\n", |
| 3369 | + " font-size: 20px;\n", |
| 3370 | + " }\n", |
| 3371 | + " .flip-card-back-q2 {\n", |
| 3372 | + " background-color: #a8d480;\n", |
| 3373 | + " transform: rotateY(180deg);\n", |
| 3374 | + " font-size: 20px;\n", |
| 3375 | + " }\n", |
| 3376 | + " </style>\n", |
| 3377 | + "\n", |
| 3378 | + " <div class=\"flip-card-q2\">\n", |
| 3379 | + " <div class=\"flip-card-inner-q2\">\n", |
| 3380 | + " <div class=\"flip-card-front-q2\">\n", |
| 3381 | + " What properties does a productive AIR have?\n", |
| 3382 | + " </div>\n", |
| 3383 | + " <div class=\"flip-card-back-q2\">\n", |
| 3384 | + " It spans over the V- to the J-gene with a CDR3 regions contained. It contains a start codon in the leading region.\n", |
| 3385 | + " </div>\n", |
| 3386 | + " </div>\n", |
| 3387 | + " </div>\n", |
| 3388 | + " <noscript>\n", |
| 3389 | + " <p><strong>Q:</strong> What properties does a productive AIR have?</p>\n", |
| 3390 | + " <p><strong>Answer:</strong> It spans over the V- to the J-gene with a CDR3 regions contained. It contains a start codon in the leading region.</p>\n", |
| 3391 | + " </noscript>\n", |
| 3392 | + " " |
| 3393 | + ], |
| 3394 | + "text/plain": [ |
| 3395 | + "<IPython.core.display.HTML object>" |
| 3396 | + ] |
| 3397 | + }, |
| 3398 | + "metadata": {}, |
| 3399 | + "output_type": "display_data" |
| 3400 | + }, |
| 3401 | + { |
| 3402 | + "data": { |
| 3403 | + "text/html": [ |
| 3404 | + "\n", |
| 3405 | + " <style>\n", |
| 3406 | + " div#flip-card .output.text_html {\n", |
| 3407 | + " background-color: transparent;\n", |
| 3408 | + " }\n", |
| 3409 | + " .flip-card-q3 {\n", |
| 3410 | + " background-color: transparent;\n", |
| 3411 | + " width: 350px;\n", |
| 3412 | + " height: 200px;\n", |
| 3413 | + " perspective: 1000px;\n", |
| 3414 | + " display: inline-block;\n", |
| 3415 | + " margin: 10px;\n", |
| 3416 | + " text-align: center;\n", |
| 3417 | + " padding: 10px;\n", |
| 3418 | + " }\n", |
| 3419 | + " .flip-card-inner-q3 {\n", |
| 3420 | + " position: relative;\n", |
| 3421 | + " width: 100%;\n", |
| 3422 | + " height: 100%;\n", |
| 3423 | + " text-align: center;\n", |
| 3424 | + " transition: transform 0.6s;\n", |
| 3425 | + " transform-style: preserve-3d;\n", |
| 3426 | + " transform-origin: center;\n", |
| 3427 | + " }\n", |
| 3428 | + " .flip-card-q3:hover .flip-card-inner-q3 {\n", |
| 3429 | + " transform: rotateY(180deg);\n", |
| 3430 | + " }\n", |
| 3431 | + " .flip-card-front-q3, .flip-card-back-q3 {\n", |
| 3432 | + " position: absolute;\n", |
| 3433 | + " width: 100%;\n", |
| 3434 | + " height: 100%;\n", |
| 3435 | + " min-height: 200px;\n", |
| 3436 | + " backface-visibility: hidden;\n", |
| 3437 | + " display: flex;\n", |
| 3438 | + " align-items: center;\n", |
| 3439 | + " justify-content: center;\n", |
| 3440 | + " font-weight: bold;\n", |
| 3441 | + " color: white;\n", |
| 3442 | + " padding: 20px;\n", |
| 3443 | + " border-radius: 15px;\n", |
| 3444 | + " box-sizing: border-box;\n", |
| 3445 | + " overflow: hidden;\n", |
| 3446 | + " text-align: center;\n", |
| 3447 | + " }\n", |
| 3448 | + " .flip-card-front-q3 {\n", |
| 3449 | + " background-color: #3965a3;\n", |
| 3450 | + " font-size: 20px;\n", |
| 3451 | + " }\n", |
| 3452 | + " .flip-card-back-q3 {\n", |
| 3453 | + " background-color: #a8d480;\n", |
| 3454 | + " transform: rotateY(180deg);\n", |
| 3455 | + " font-size: 20px;\n", |
| 3456 | + " }\n", |
| 3457 | + " </style>\n", |
| 3458 | + "\n", |
| 3459 | + " <div class=\"flip-card-q3\">\n", |
| 3460 | + " <div class=\"flip-card-inner-q3\">\n", |
| 3461 | + " <div class=\"flip-card-front-q3\">\n", |
| 3462 | + " What cells should be identified for potential filtering?\n", |
| 3463 | + " </div>\n", |
| 3464 | + " <div class=\"flip-card-back-q3\">\n", |
| 3465 | + " Cells with high count of AIR sequence reads, cells with incomplete AIR information, etc.\n", |
| 3466 | + " </div>\n", |
| 3467 | + " </div>\n", |
| 3468 | + " </div>\n", |
| 3469 | + " <noscript>\n", |
| 3470 | + " <p><strong>Q:</strong> What cells should be identified for potential filtering?</p>\n", |
| 3471 | + " <p><strong>Answer:</strong> Cells with high count of AIR sequence reads, cells with incomplete AIR information, etc.</p>\n", |
| 3472 | + " </noscript>\n", |
| 3473 | + " " |
| 3474 | + ], |
| 3475 | + "text/plain": [ |
| 3476 | + "<IPython.core.display.HTML object>" |
| 3477 | + ] |
| 3478 | + }, |
| 3479 | + "metadata": {}, |
| 3480 | + "output_type": "display_data" |
| 3481 | + } |
| 3482 | + ], |
| 3483 | + "source": [ |
| 3484 | + "%run ../src/lib.py\n", |
3239 | 3485 | "\n", |
3240 | | - "What cells should be identified for potential filtering?\n", |
3241 | | - "- Cells with a high count of AIR sequence reads.\n", |
3242 | | - "- \\+ Cells with incomplete AIR information.\n", |
3243 | | - "- \\+ Cells with multiple AIRs indicating doublets.\n", |
3244 | | - "- Cells with specific motifs in the CDR3 region." |
| 3486 | + "flip_card(\n", |
| 3487 | + " \"q1\",\n", |
| 3488 | + " \"Where does the high variability of AIRs stem from?\",\n", |
| 3489 | + " \"Junctional diversity adds random nucleotides. Peripheral diversity motivates rapid amino acid switches specific to the host organ. Combinatorial diversity of different gene segments. Inherited diversity genetically encodes multiple AIRs.\",\n", |
| 3490 | + " back_font_size=13,\n", |
| 3491 | + ")\n", |
| 3492 | + "flip_card(\n", |
| 3493 | + " \"q2\",\n", |
| 3494 | + " \"What properties does a productive AIR have?\",\n", |
| 3495 | + " \"It spans over the V- to the J-gene with a CDR3 regions contained. It contains a start codon in the leading region.\",\n", |
| 3496 | + ")\n", |
| 3497 | + "flip_card(\n", |
| 3498 | + " \"q3\",\n", |
| 3499 | + " \"What cells should be identified for potential filtering?\",\n", |
| 3500 | + " \"Cells with high count of AIR sequence reads, cells with incomplete AIR information, etc.\",\n", |
| 3501 | + ")" |
3245 | 3502 | ] |
3246 | 3503 | }, |
3247 | 3504 | { |
|
3265 | 3522 | ], |
3266 | 3523 | "metadata": { |
3267 | 3524 | "kernelspec": { |
3268 | | - "display_name": "Python 3.10.5 64-bit", |
| 3525 | + "display_name": "book", |
3269 | 3526 | "language": "python", |
3270 | 3527 | "name": "python3" |
3271 | 3528 | }, |
|
3279 | 3536 | "name": "python", |
3280 | 3537 | "nbconvert_exporter": "python", |
3281 | 3538 | "pygments_lexer": "ipython3", |
3282 | | - "version": "3.10.8" |
3283 | | - }, |
3284 | | - "vscode": { |
3285 | | - "interpreter": { |
3286 | | - "hash": "e7370f93d1d0cde622a1f8e1c04877d8463912d04d973331ad4851f04de6915a" |
3287 | | - } |
| 3539 | + "version": "3.11.8" |
3288 | 3540 | } |
3289 | 3541 | }, |
3290 | 3542 | "nbformat": 4, |
|
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