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Hi!
Sorry if this is lame, desperate biologist here.
I still have painful blanks regarding the proper usage of layers in v5. I dream about my beloved v4, but cannot go back - my collabs use v5 and leave me no choice.
For example all the assay=SCT/RNA parameters??? E.g., in Dotplots and whatnot? "Assay=RNA" is also still included in the 2024 pseudobulk vignette, which I thought was written up in a v5 context...
Does anyone know good resources or threads where this has been discussed thoroughly? Or anything that might help? Thanks very much, and again..sorry!
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Hi!
Sorry if this is lame, desperate biologist here.
I still have painful blanks regarding the proper usage of layers in v5. I dream about my beloved v4, but cannot go back - my collabs use v5 and leave me no choice.
For example all the assay=SCT/RNA parameters??? E.g., in Dotplots and whatnot? "Assay=RNA" is also still included in the 2024 pseudobulk vignette, which I thought was written up in a v5 context...
Does anyone know good resources or threads where this has been discussed thoroughly? Or anything that might help? Thanks very much, and again..sorry!
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