Help with the pooled mode

[WatanyaTra]

Hi,

I have a problem using the pooled mode.

I ran the command below, and it gave an error that "Incorrect number of columns provided without header."

CRISPRessoPooled -r1 hFANCF_R1_001.fastq.gz -r2 hFANCF_R2_001.fastq.gz -f hFANCF_BatchFile.txt -q 30 --name hFANCF
hFANCF_BatchFile.txt

My batch file has 14 columns, and I have no idea why it gave that error. Does anyone know how to fix this issue? Thank you!

Best wishes,
Tanya

[Colelyman]

Hi @WatanyaTra,

Thanks for using CRISPResso! Sorry to hear that you are having trouble. After looking at the hFANCF_BatchFile.txt and trying to run it using version 2.3.2, it looks like everything is correct. Would you mind sharing which version you are using? And if you still get an error after using version 2.3.2, could you run with the --debug parameter and share the full output?

Thanks,
Cole