diff --git a/package-lock.json b/package-lock.json
index 4dd4b0bc05..4979d0f992 100644
--- a/package-lock.json
+++ b/package-lock.json
@@ -8959,9 +8959,9 @@
       }
     },
     "node_modules/@opentelemetry/semantic-conventions": {
-      "version": "1.33.0",
-      "resolved": "https://registry.npmjs.org/@opentelemetry/semantic-conventions/-/semantic-conventions-1.33.0.tgz",
-      "integrity": "sha512-TIpZvE8fiEILFfTlfPnltpBaD3d9/+uQHVCyC3vfdh6WfCXKhNFzoP5RyDDIndfvZC5GrA4pyEDNyjPloJud+w==",
+      "version": "1.33.1",
+      "resolved": "https://registry.npmjs.org/@opentelemetry/semantic-conventions/-/semantic-conventions-1.33.1.tgz",
+      "integrity": "sha512-tBco+nVMoGqJ0LHPKOWlN9o1PX2AA9zxDMgvL210YYXlG9UkMXpiXhsWvvQriZrjOzTXXEpH6jleCgAuVGg3EQ==",
       "engines": {
         "node": ">=14"
       }
@@ -36338,8 +36338,8 @@
       "version": "0.46.0",
       "license": "Apache-2.0",
       "dependencies": {
-        "@opentelemetry/instrumentation": "^0.201.0",
-        "@opentelemetry/semantic-conventions": "^1.27.0"
+        "@opentelemetry/instrumentation": "^0.201.1",
+        "@opentelemetry/semantic-conventions": "^1.33.1"
       },
       "devDependencies": {
         "@opentelemetry/api": "^1.3.0",
@@ -36362,6 +36362,37 @@
         "@opentelemetry/api": "^1.3.0"
       }
     },
+    "plugins/node/opentelemetry-instrumentation-knex/node_modules/@opentelemetry/api-logs": {
+      "version": "0.201.1",
+      "resolved": "https://registry.npmjs.org/@opentelemetry/api-logs/-/api-logs-0.201.1.tgz",
+      "integrity": "sha512-IxcFDP1IGMDemVFG2by/AMK+/o6EuBQ8idUq3xZ6MxgQGeumYZuX5OwR0h9HuvcUc/JPjQGfU5OHKIKYDJcXeA==",
+      "license": "Apache-2.0",
+      "dependencies": {
+        "@opentelemetry/api": "^1.3.0"
+      },
+      "engines": {
+        "node": ">=8.0.0"
+      }
+    },
+    "plugins/node/opentelemetry-instrumentation-knex/node_modules/@opentelemetry/instrumentation": {
+      "version": "0.201.1",
+      "resolved": "https://registry.npmjs.org/@opentelemetry/instrumentation/-/instrumentation-0.201.1.tgz",
+      "integrity": "sha512-6EOSoT2zcyBM3VryAzn35ytjRrOMeaWZyzQ/PHVfxoXp5rMf7UUgVToqxOhQffKOHtC7Dma4bHt+DuwIBBZyZA==",
+      "license": "Apache-2.0",
+      "dependencies": {
+        "@opentelemetry/api-logs": "0.201.1",
+        "@types/shimmer": "^1.2.0",
+        "import-in-the-middle": "^1.8.1",
+        "require-in-the-middle": "^7.1.1",
+        "shimmer": "^1.2.1"
+      },
+      "engines": {
+        "node": "^18.19.0 || >=20.6.0"
+      },
+      "peerDependencies": {
+        "@opentelemetry/api": "^1.3.0"
+      }
+    },
     "plugins/node/opentelemetry-instrumentation-knex/node_modules/@types/node": {
       "version": "18.18.14",
       "resolved": "https://registry.npmjs.org/@types/node/-/node-18.18.14.tgz",
@@ -36371,6 +36402,12 @@
         "undici-types": "~5.26.4"
       }
     },
+    "plugins/node/opentelemetry-instrumentation-knex/node_modules/@types/shimmer": {
+      "version": "1.2.0",
+      "resolved": "https://registry.npmjs.org/@types/shimmer/-/shimmer-1.2.0.tgz",
+      "integrity": "sha512-UE7oxhQLLd9gub6JKIAhDq06T0F6FnztwMNRvYgjeQSBeMc1ZG/tA47EwfduvkuQS8apbkM/lpLpWsaCeYsXVg==",
+      "license": "MIT"
+    },
     "plugins/node/opentelemetry-instrumentation-knex/node_modules/typescript": {
       "version": "5.0.4",
       "resolved": "https://registry.npmjs.org/typescript/-/typescript-5.0.4.tgz",
@@ -45218,10 +45255,10 @@
       "requires": {
         "@opentelemetry/api": "^1.3.0",
         "@opentelemetry/context-async-hooks": "^2.0.0",
-        "@opentelemetry/instrumentation": "^0.201.0",
+        "@opentelemetry/instrumentation": "^0.201.1",
         "@opentelemetry/sdk-trace-base": "^2.0.0",
         "@opentelemetry/sdk-trace-node": "^2.0.0",
-        "@opentelemetry/semantic-conventions": "^1.27.0",
+        "@opentelemetry/semantic-conventions": "^1.33.1",
         "@types/mocha": "10.0.10",
         "@types/node": "18.18.14",
         "better-sqlite3": "11.0.0",
@@ -45232,6 +45269,26 @@
         "typescript": "5.0.4"
       },
       "dependencies": {
+        "@opentelemetry/api-logs": {
+          "version": "0.201.1",
+          "resolved": "https://registry.npmjs.org/@opentelemetry/api-logs/-/api-logs-0.201.1.tgz",
+          "integrity": "sha512-IxcFDP1IGMDemVFG2by/AMK+/o6EuBQ8idUq3xZ6MxgQGeumYZuX5OwR0h9HuvcUc/JPjQGfU5OHKIKYDJcXeA==",
+          "requires": {
+            "@opentelemetry/api": "^1.3.0"
+          }
+        },
+        "@opentelemetry/instrumentation": {
+          "version": "0.201.1",
+          "resolved": "https://registry.npmjs.org/@opentelemetry/instrumentation/-/instrumentation-0.201.1.tgz",
+          "integrity": "sha512-6EOSoT2zcyBM3VryAzn35ytjRrOMeaWZyzQ/PHVfxoXp5rMf7UUgVToqxOhQffKOHtC7Dma4bHt+DuwIBBZyZA==",
+          "requires": {
+            "@opentelemetry/api-logs": "0.201.1",
+            "@types/shimmer": "^1.2.0",
+            "import-in-the-middle": "^1.8.1",
+            "require-in-the-middle": "^7.1.1",
+            "shimmer": "^1.2.1"
+          }
+        },
         "@types/node": {
           "version": "18.18.14",
           "resolved": "https://registry.npmjs.org/@types/node/-/node-18.18.14.tgz",
@@ -45241,6 +45298,11 @@
             "undici-types": "~5.26.4"
           }
         },
+        "@types/shimmer": {
+          "version": "1.2.0",
+          "resolved": "https://registry.npmjs.org/@types/shimmer/-/shimmer-1.2.0.tgz",
+          "integrity": "sha512-UE7oxhQLLd9gub6JKIAhDq06T0F6FnztwMNRvYgjeQSBeMc1ZG/tA47EwfduvkuQS8apbkM/lpLpWsaCeYsXVg=="
+        },
         "typescript": {
           "version": "5.0.4",
           "resolved": "https://registry.npmjs.org/typescript/-/typescript-5.0.4.tgz",
@@ -47558,9 +47620,9 @@
       }
     },
     "@opentelemetry/semantic-conventions": {
-      "version": "1.33.0",
-      "resolved": "https://registry.npmjs.org/@opentelemetry/semantic-conventions/-/semantic-conventions-1.33.0.tgz",
-      "integrity": "sha512-TIpZvE8fiEILFfTlfPnltpBaD3d9/+uQHVCyC3vfdh6WfCXKhNFzoP5RyDDIndfvZC5GrA4pyEDNyjPloJud+w=="
+      "version": "1.33.1",
+      "resolved": "https://registry.npmjs.org/@opentelemetry/semantic-conventions/-/semantic-conventions-1.33.1.tgz",
+      "integrity": "sha512-tBco+nVMoGqJ0LHPKOWlN9o1PX2AA9zxDMgvL210YYXlG9UkMXpiXhsWvvQriZrjOzTXXEpH6jleCgAuVGg3EQ=="
     },
     "@opentelemetry/sql-common": {
       "version": "file:packages/opentelemetry-sql-common",
diff --git a/plugins/node/opentelemetry-instrumentation-knex/README.md b/plugins/node/opentelemetry-instrumentation-knex/README.md
index 596fdcc619..be61ddce58 100644
--- a/plugins/node/opentelemetry-instrumentation-knex/README.md
+++ b/plugins/node/opentelemetry-instrumentation-knex/README.md
@@ -54,21 +54,38 @@ registerInstrumentations({
 
 ## Semantic Conventions
 
-This package uses `@opentelemetry/semantic-conventions` version `1.22+`, which implements Semantic Convention [Version 1.7.0](https://github.com/open-telemetry/opentelemetry-specification/blob/v1.7.0/semantic_conventions/README.md)
+This package uses `@opentelemetry/semantic-conventions` version `1.33+`, which implements Semantic Convention [Version 1.7.0](https://github.com/open-telemetry/opentelemetry-specification/blob/v1.7.0/semantic_conventions/README.md)
+
+This package is capable of emitting both Semantic Convention [Version 1.7.0](https://github.com/open-telemetry/opentelemetry-specification/blob/v1.7.0/semantic_conventions/README.md) and [Version 1.33.0](https://github.com/open-telemetry/semantic-conventions/blob/v1.33.0/docs/database/database-spans.md)
+It is controlled using the environment variable `OTEL_SEMCONV_STABILITY_OPT_IN`, which is a comma separated list of values.
+The values `database` and `database/dup` control this instrumentation.
+See details for the behavior of each of these values below.
+If neither `database` or `database/dup` is included in `OTEL_SEMCONV_STABILITY_OPT_IN`, the old experimental semantic conventions will be used by default.
+
+### Upgrading Semantic Conventions
+
+When upgrading to the new semantic conventions, it is recommended to do so in the following order:
+
+1. Upgrade `@opentelemetry/instrumentation-knex` to the latest version
+2. Set `OTEL_SEMCONV_STABILITY_OPT_IN=database/dup` to emit both old and new semantic conventions
+3. Modify alerts, dashboards, metrics, and other processes to expect the new semantic conventions
+4. Set `OTEL_SEMCONV_STABILITY_OPT_IN=database` to emit only the new semantic conventions
+
+This will cause both the old and new semantic conventions to be emitted during the transition period.
 
 Attributes collected:
 
-| Attribute               | Short Description                                                              |
-| ----------------------- | ------------------------------------------------------------------------------ |
-| `db.name`               | This attribute is used to report the name of the database being accessed.      |
-| `db.operation`          | The name of the operation being executed.                                      |
-| `db.sql.table`          | The name of the primary table that the operation is acting upon.               |
-| `db.statement`          | The database statement being executed.                                         |
-| `db.system`             | An identifier for the database management system (DBMS) product being used.    |
-| `db.user`               | Username for accessing the database.                                           |
-| `net.peer.name`         | Remote hostname or similar.                                                    |
-| `net.peer.port`         | Remote port number.                                                            |
-| `net.transport`         | Transport protocol used.                                                       |
+| v1.7.0 semconv  | v1.23.0 semconv      | Short Description                                                           |
+|-----------------|----------------------|-----------------------------------------------------------------------------|
+| `db.name`       | `db.namespace`       | This attribute is used to report the name of the database being accessed.   |
+| `db.operation`  | `db.operation.name`  | The name of the operation being executed.                                   |
+| `db.sql.table`  | `db.collection.name` | The name of the primary table that the operation is acting upon.            |
+| `db.statement`  | `db.query.text`      | The database statement being executed.                                      |
+| `db.system`     | `db.system.name`     | An identifier for the database management system (DBMS) product being used. |
+| `db.user`       | (not included)       | Username for accessing the database.                                        |
+| `net.peer.name` | `server.address`     | Remote hostname or similar.                                                 |
+| `net.peer.port` | `server.port`        | Remote port number.                                                         |
+| `net.transport` | (not included)       | Transport protocol used.                                                    |
 
 ## Useful links
 
diff --git a/plugins/node/opentelemetry-instrumentation-knex/package.json b/plugins/node/opentelemetry-instrumentation-knex/package.json
index 3d8a5dab39..47710a914e 100644
--- a/plugins/node/opentelemetry-instrumentation-knex/package.json
+++ b/plugins/node/opentelemetry-instrumentation-knex/package.json
@@ -56,8 +56,8 @@
     "typescript": "5.0.4"
   },
   "dependencies": {
-    "@opentelemetry/instrumentation": "^0.201.0",
-    "@opentelemetry/semantic-conventions": "^1.27.0"
+    "@opentelemetry/instrumentation": "^0.201.1",
+    "@opentelemetry/semantic-conventions": "^1.33.1"
   },
   "homepage": "https://github.com/open-telemetry/opentelemetry-js-contrib/tree/main/plugins/node/opentelemetry-instrumentation-knex#readme"
 }
diff --git a/plugins/node/opentelemetry-instrumentation-knex/src/instrumentation.ts b/plugins/node/opentelemetry-instrumentation-knex/src/instrumentation.ts
index 5b7086895d..bc13842a77 100644
--- a/plugins/node/opentelemetry-instrumentation-knex/src/instrumentation.ts
+++ b/plugins/node/opentelemetry-instrumentation-knex/src/instrumentation.ts
@@ -23,8 +23,19 @@ import {
   InstrumentationNodeModuleDefinition,
   InstrumentationNodeModuleFile,
   isWrapped,
+  SemconvStability,
+  semconvStabilityFromStr,
 } from '@opentelemetry/instrumentation';
+import * as utils from './utils';
+import { KnexInstrumentationConfig } from './types';
 import {
+  ATTR_DB_COLLECTION_NAME,
+  ATTR_DB_NAMESPACE,
+  ATTR_DB_OPERATION_NAME,
+  ATTR_DB_QUERY_TEXT,
+  ATTR_DB_SYSTEM_NAME,
+  ATTR_SERVER_ADDRESS,
+  ATTR_SERVER_PORT,
   SEMATTRS_DB_NAME,
   SEMATTRS_DB_OPERATION,
   SEMATTRS_DB_SQL_TABLE,
@@ -35,8 +46,6 @@ import {
   SEMATTRS_NET_PEER_PORT,
   SEMATTRS_NET_TRANSPORT,
 } from '@opentelemetry/semantic-conventions';
-import * as utils from './utils';
-import { KnexInstrumentationConfig } from './types';
 
 const contextSymbol = Symbol('opentelemetry.instrumentation-knex.context');
 const DEFAULT_CONFIG: KnexInstrumentationConfig = {
@@ -45,8 +54,15 @@ const DEFAULT_CONFIG: KnexInstrumentationConfig = {
 };
 
 export class KnexInstrumentation extends InstrumentationBase<KnexInstrumentationConfig> {
+  private _semconvStability: SemconvStability;
+
   constructor(config: KnexInstrumentationConfig = {}) {
     super(PACKAGE_NAME, PACKAGE_VERSION, { ...DEFAULT_CONFIG, ...config });
+
+    this._semconvStability = semconvStabilityFromStr(
+      'database',
+      process.env.OTEL_SEMCONV_STABILITY_OPT_IN
+    );
   }
 
   override setConfig(config: KnexInstrumentationConfig = {}) {
@@ -122,6 +138,7 @@ export class KnexInstrumentation extends InstrumentationBase<KnexInstrumentation
 
   private createQueryWrapper(moduleVersion?: string) {
     const instrumentation = this;
+
     return function wrapQuery(original: (...args: any[]) => any) {
       return function wrapped_logging_method(this: any, query: any) {
         const config = this.client.config;
@@ -134,24 +151,43 @@ export class KnexInstrumentation extends InstrumentationBase<KnexInstrumentation
           config?.connection?.filename || config?.connection?.database;
         const { maxQueryLength } = instrumentation.getConfig();
 
-        const attributes: api.SpanAttributes = {
+        const attributes: api.Attributes = {
           'knex.version': moduleVersion,
-          [SEMATTRS_DB_SYSTEM]: utils.mapSystem(config.client),
-          [SEMATTRS_DB_SQL_TABLE]: table,
-          [SEMATTRS_DB_OPERATION]: operation,
-          [SEMATTRS_DB_USER]: config?.connection?.user,
-          [SEMATTRS_DB_NAME]: name,
-          [SEMATTRS_NET_PEER_NAME]: config?.connection?.host,
-          [SEMATTRS_NET_PEER_PORT]: config?.connection?.port,
-          [SEMATTRS_NET_TRANSPORT]:
-            config?.connection?.filename === ':memory:' ? 'inproc' : undefined,
         };
+        const transport =
+          config?.connection?.filename === ':memory:' ? 'inproc' : undefined;
+
+        if (instrumentation._semconvStability & SemconvStability.OLD) {
+          Object.assign(attributes, {
+            [SEMATTRS_DB_SYSTEM]: utils.mapSystem(config.client),
+            [SEMATTRS_DB_SQL_TABLE]: table,
+            [SEMATTRS_DB_OPERATION]: operation,
+            [SEMATTRS_DB_USER]: config?.connection?.user,
+            [SEMATTRS_DB_NAME]: name,
+            [SEMATTRS_NET_PEER_NAME]: config?.connection?.host,
+            [SEMATTRS_NET_PEER_PORT]: config?.connection?.port,
+            [SEMATTRS_NET_TRANSPORT]: transport,
+          });
+        }
+        if (instrumentation._semconvStability & SemconvStability.STABLE) {
+          Object.assign(attributes, {
+            [ATTR_DB_SYSTEM_NAME]: utils.mapSystem(config.client),
+            [ATTR_DB_COLLECTION_NAME]: table,
+            [ATTR_DB_OPERATION_NAME]: operation,
+            [ATTR_DB_NAMESPACE]: name,
+            [ATTR_SERVER_ADDRESS]: config?.connection?.host,
+            [ATTR_SERVER_PORT]: config?.connection?.port,
+          });
+        }
         if (maxQueryLength) {
           // filters both undefined and 0
-          attributes[SEMATTRS_DB_STATEMENT] = utils.limitLength(
-            query?.sql,
-            maxQueryLength
-          );
+          const queryText = utils.limitLength(query?.sql, maxQueryLength);
+          if (instrumentation._semconvStability & SemconvStability.STABLE) {
+            attributes[ATTR_DB_QUERY_TEXT] = queryText;
+          }
+          if (instrumentation._semconvStability & SemconvStability.OLD) {
+            attributes[SEMATTRS_DB_STATEMENT] = queryText;
+          }
         }
 
         const parentContext =
diff --git a/plugins/node/opentelemetry-instrumentation-knex/src/semconv.ts b/plugins/node/opentelemetry-instrumentation-knex/src/semconv.ts
new file mode 100644
index 0000000000..f2a9b5043d
--- /dev/null
+++ b/plugins/node/opentelemetry-instrumentation-knex/src/semconv.ts
@@ -0,0 +1,26 @@
+/*
+ * Copyright The OpenTelemetry Authors
+ *
+ * Licensed under the Apache License, Version 2.0 (the "License");
+ * you may not use this file except in compliance with the License.
+ * You may obtain a copy of the License at
+ *
+ *      https://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS,
+ * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+ * See the License for the specific language governing permissions and
+ * limitations under the License.
+ */
+
+/*
+ * This file contains a copy of unstable semantic convention definitions
+ * used by this package.
+ * @see https://github.com/open-telemetry/opentelemetry-js/tree/main/semantic-conventions#unstable-semconv
+ */
+
+/**
+ * Enum value "sqlite" for attribute {@link ATTR_DB_SYSTEM_NAME}.
+ */
+export const DB_SYSTEM_NAME_VALUE_SQLITE = 'sqlite' as const;
diff --git a/plugins/node/opentelemetry-instrumentation-knex/src/utils.ts b/plugins/node/opentelemetry-instrumentation-knex/src/utils.ts
index 1ef06eb6bf..38fbcce5e3 100644
--- a/plugins/node/opentelemetry-instrumentation-knex/src/utils.ts
+++ b/plugins/node/opentelemetry-instrumentation-knex/src/utils.ts
@@ -15,10 +15,8 @@
  */
 
 import { Exception } from '@opentelemetry/api';
-import {
-  DBSYSTEMVALUES_SQLITE,
-  DBSYSTEMVALUES_POSTGRESQL,
-} from '@opentelemetry/semantic-conventions';
+import { DB_SYSTEM_NAME_VALUE_POSTGRESQL } from '@opentelemetry/semantic-conventions';
+import { DB_SYSTEM_NAME_VALUE_SQLITE } from './semconv';
 
 type KnexError = Error & {
   code?: string;
@@ -57,8 +55,8 @@ export function otelExceptionFromKnexError(
 }
 
 const systemMap = new Map([
-  ['sqlite3', DBSYSTEMVALUES_SQLITE],
-  ['pg', DBSYSTEMVALUES_POSTGRESQL],
+  ['sqlite3', DB_SYSTEM_NAME_VALUE_SQLITE],
+  ['pg', DB_SYSTEM_NAME_VALUE_POSTGRESQL],
 ]);
 
 export const mapSystem = (knexSystem: string) => {