Skip to content

funannotate annotate fails at the BUSCO annotation step #1108

New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Open
SM-2797 opened this issue May 20, 2025 · 1 comment
Open

funannotate annotate fails at the BUSCO annotation step #1108

SM-2797 opened this issue May 20, 2025 · 1 comment

Comments

@SM-2797
Copy link

SM-2797 commented May 20, 2025

I am computing functional annotations based on precomputed eggnog and interproscan annotations and using the BUSCO dataset "eukaryota". The funannotate database folder contains the required BUSCO dataset, but the "funannotate annotate" command fails at the BUSCO step with the error below:

: Annotating proteins with BUSCO eukaryota models
Traceback (most recent call last):
File "/.conda/envs/funannotate/bin/funannotate", line 10, in
sys.exit(main())
File "/.conda/envs/funannotate/lib/python3.9/site-packages/funannotate/funannotate.py", line 717, in main
mod.main(arguments)
File "/.conda/envs/funannotate/lib/python3.9/site-packages/funannotate/annotate.py", line 1278, in main
lib.runBUSCO(
File "/.conda/envs/funannotate/lib/python3.9/site-packages/funannotate/library.py", line 6893, in runBUSCO
with open(
FileNotFoundError: [Errno 2] No such file or directory: 'annot_output/annotate_misc/run_busco/full_table_busco.tsv'

Any idea what might be the problem?

Thanks.

@hyphaltip
Copy link
Collaborator

would need to see what other errors but usually the busco sets include the version number (eg eukaryota_odb10) - make sure that also exists in the $FUNANNOTATE_DB folder as well.

can you provide the cmdline you are using to run annotate as well?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants