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Copy file name to clipboardExpand all lines: Microarray/Affymetrix/Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md
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> **This page holds an overview and instructions for how GeneLab processes Affymetrix microarray datasets. Exact processing commands and GL-DPPD-7114 version used for specific GeneLab datasets (GLDS) are provided with their processed data in the [Open Science Data
> \* The pipeline detailed below is currently used for animal studies only, it will be updated soon for processing plants and microbe microarray data.
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> \* The pipeline detailed below is currently used for animal and Arabidopsis Thaliana studies only, it will be updated soon for processing microbe microarray data and other plant data.
- Support for Arabidposis Thaliana datasets using the plants ensembl FTP server.
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- Support for raw data FeatureSets (building on existing support for ExpressionSets)
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- Better support for non-ascii characters in the runsheet, usually caused by such characters in the original ISA archive the runsheet is generated from.
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### Fixed
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- Typos related to shared code with Agilent 1 Channel platform.
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### Changed
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- Error message when encountering unique columns when reordering tables is now clearer about what unique columns were found.
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- Post Processing Workflow: Assay Table Update now added '_array_' prefix to processed files instead of '_microarray_' prefix.
Copy file name to clipboardExpand all lines: Microarray/Affymetrix/Workflow_Documentation/NF_MAAffymetrix/README.md
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copy of latest NF_MAAffymetrix version on to your system, the code can be downloaded as a zip file from the release page then unzipped after downloading by running the following commands:
While in the location containing the `NF_MAAffymetrix_1.0.0` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_MAAffymetrix workflow:
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While in the location containing the `NF_MAAffymetrix_1.0.1` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_MAAffymetrix workflow:
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> Note: Nextflow commands use both single hyphen arguments (e.g. -help) that denote general nextflow arguments and double hyphen arguments (e.g. --ensemblVersion) that denote workflow specific parameters. Take care to use the proper number of hyphens for each argument.
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<br>
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#### 3a. Approach 1: Run the workflow on a GeneLab Affymetrix Microarray dataset
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```bash
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nextflow run NF_MAAffymetrix_1.0.0/main.nf \
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nextflow run NF_MAAffymetrix_1.0.1/main.nf \
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-profile singularity \
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--osdAccession OSD-266 \
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--gldsAccession GLDS-266
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> Note: Specifications for creating a runsheet manually are described [here](examples/runsheet/README.md).
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```bash
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nextflow run NF_MAAffymetrix_1.0.0/main.nf \
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nextflow run NF_MAAffymetrix_1.0.1/main.nf \
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-profile singularity \
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--runsheetPath </path/to/runsheet>
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```
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**Required Parameters For All Approaches:**
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* `NF_MAAffymetrix_1.0.0/main.nf` - Instructs Nextflow to run the NF_MAAffymetrix workflow
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* `NF_MAAffymetrix_1.0.1/main.nf` - Instructs Nextflow to run the NF_MAAffymetrix workflow
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* `-profile` - Specifies the configuration profile(s) to load, `singularity` instructs Nextflow to setup and use singularity for all software called in the workflow
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All parameters listed above and additional optional arguments for the NF_MAAffymetrix workflow, including debug related options that may not be immediately useful for most users, can be viewed by running the following command:
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```bash
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nextflow run NF_MAAffymetrix_1.0.0/main.nf --help
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nextflow run NF_MAAffymetrix_1.0.1/main.nf --help
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```
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See `nextflow run -h` and [Nextflow's CLI run command documentation](https://nextflow.io/docs/latest/cli.html#run) for more options and details common to all nextflow workflows.
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All R code steps and output are rendered within a Quarto document yielding the following:
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- Output:
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- NF_MAAffymetrix_1.0.0.html (html report containing executed code and output including QA plots)
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- NF_MAAffymetrix_1.0.1.html (html report containing executed code and output including QA plots)
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The outputs from the Analysis Staging and V&V Pipeline Subworkflows are described below:
GeneLab has wrapped each step of the pipeline into a workflow with validation and verification of output files built in after each step. The table below lists (and links to) each NF_MAAffymetrix version and the corresponding workflow subdirectory, the current NF_MAAffymetrix/workflow implementation is indicated. Each workflow subdirectory contains information about the workflow along with instructions for installation and usage.**
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> **The document [`GL-DPPD-7114.md`](Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md) holds an overview and example commands for how GeneLab processes Affymetrix microarray datasets. See the [Repository Links](#repository-links) descriptions below for more information. Processed data output files and processing code is provided for each GLDS dataset along with the processed data in the [GeneLab Data Systems (GLDS) repository](https://genelab-data.ndc.nasa.gov/genelab/projects).**
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## NF_MAAffymetrix Version and Corresponding Workflow
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---
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|Pipeline Version|Current Workflow Version (for respective pipeline version)|Nextflow Version|
- Contains instructions for installing and running the GeneLab NF_MAAffymetrix workflow
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---
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**Developed and maintained by:**
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Jonathan Oribello
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> See the [workflow changelog](NF_MAAffymetrix/CHANGELOG.md) to access previous workflow versions and view all changes associated with each version update.
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