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Amplicon/Illumina/Workflow_Documentation/NF_AmpIllumina/README.md renamed to Amplicon/Illumina/Workflow_Documentation/NF_AmpIllumina-B/README.md

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@@ -111,7 +111,7 @@ nextflow run main.nf --help
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#### 4a. Approach 1: Run slurm jobs in singularity containers with OSD accession as input
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```bash
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nextflow run main.nf -resume -profile slurm,singularity --GLDS_accession GLDS-487 --target_region 16S
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nextflow run main.nf -resume -profile slurm,singularity --accession GLDS-487 --target_region 16S
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```
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<br>
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*Required only if you would like to pull and process data directly from OSDR*
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* `--GLDS_accession` – A Genelab / OSD accession number e.g. GLDS-487.
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* `--accession` – A Genelab / OSD accession number e.g. GLDS-487.
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*Required only if --GLDS_accession is not passed as an argument*
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*Required only if --accession is not passed as an argument*
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* `--csv_file` – A 3-column (single-end) or 4-column (paired-end) input csv file (sample_id, forward, [reverse,] paired). Please see the sample [SE_file.csv](workflow_code/SE_file.csv) and [PE_file.csv](workflow_code/PE_file.csv) in this repository for examples on how to format this file.
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@@ -200,4 +200,20 @@ To generate a README file, a protocols file, a md5sums table and a file associat
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nextflow -C post_processing.config run post_processng.nf -resume -profile slurm,singularity
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```
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The outputs of the run will be in a directory called `Post_Processing` by default.
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The outputs of the run will be in a directory called `Post_Processing` by default and they are as follows:
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- Post_processing/FastQC_Outputs/filtered_multiqc_GLAmpSeq_report.zip (Filtered sequence multiqc report with paths purged)
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- Post_processing/FastQC_Outputs/raw_multiqc_GLAmpSeq_report.zip (Raw sequence multiqc report with paths purged)
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- Post_processing/<GLDS_accession>_-associated-file-names.tsv (File association table for curation)
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- Post_processing/<GLDS_accession>_amplicon-validation.log (Automatic verification and validation log file)
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- Post_processing/processed_md5sum_GLAmpSeq.tsv (md5sums for the files to be released on OSDR)
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- Post_processing/processing_info_GLAmpSeq.zip (Zip file containing all files used to run the workflow and required logs with paths purged)
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- Post_processing/protocol.txt (File describing the methods used by the workflow)
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- Post_processing/README_GLAmpSeq.txt (README file listing and describing the outputs of the workflow)

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