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Additionally, the `RColorBrewer_Palette` variable can be modified in the script. This variable determines the color palette from the RColorBrewer package that is applied to the plots.
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<br>
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___
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### 3. Parameter definitions
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**Parameter definitions for Illumina-R-visualizations.R:**
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*`runsheet_file` – specifies the runsheet containing sample metadata required for processing (output from [GL-DPPD-7104-B step 6a](https://github.com/nasa/GeneLab_Data_Processing/blob/master/Amplicon/Illumina/Pipeline_GL-DPPD-7104_Versions/GL-DPPD-7104-B.md#6a-create-sample-runsheet))
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*`sample_info` – specifies the text file containing the IDs of each sample used, required for running the SW_AmpIllumina workflow (output from [run_workflow.py](/Amplicon/Illumina/Workflow_Documentation/SW_AmpIllumina-B/README.md#5-additional-output-files))
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*`counts` – specifies the ASV counts table (output from [GL-DPPD-7104-B step 5g](https://github.com/nasa/GeneLab_Data_Processing/blob/master/Amplicon/Illumina/Pipeline_GL-DPPD-7104_Versions/GL-DPPD-7104-B.md#5g-generating-and-writing-standard-outputs))
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*`taxonomy` – specifies the taxonomy table (output from [GL-DPPD-7104-B step 5g](https://github.com/nasa/GeneLab_Data_Processing/blob/master/Amplicon/Illumina/Pipeline_GL-DPPD-7104_Versions/GL-DPPD-7104-B.md#5g-generating-and-writing-standard-outputs))
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*`assay_suffix` – specifies a string that is prepended to the start of the output file names. Default: ""
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**Parameter Definitions:**
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*`runsheet_file` – specifies the table containing sample metadata required for processing
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*`sample_info` – specifies the text file containing the IDs of each sample used, required for running the SW_AmpIllumina workflow
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*`counts` – specifies the ASV counts table
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*`taxonomy` – specifies the taxonomy table
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*`assay_suffix` – specifies a string that is appended to the end of the output file names. Default: "_GLAmpSeq"
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*`plots_dir` – specifies the path where output files will be saved
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*`output_prefix` – specifies a string that is appended to the end of the output file names. Default: "_GLAmpSeq"
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*`output_prefix` – specifies a string that is prepended to the start of the output file names. Default: ""
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*`RColorBrewer_Palette` – specifies the RColorBrewer palette that will be used for coloring in the plots. Options include "Set1", "Accent", "Dark2", "Paired", "Pastel1", "Pastel2", "Set2", and "Set3". Default: "Set1"
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**Input Data:**
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**runsheet.csv (output from [GL-DPPD-7104-B step 6a](https://github.com/nasa/GeneLab_Data_Processing/blob/master/Amplicon/Illumina/Pipeline_GL-DPPD-7104_Versions/GL-DPPD-7104-B.md#6a-create-sample-runsheet))
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* unique-sample-IDs.txt (output from [run_workflow.py](/Amplicon/Illumina/Workflow_Documentation/SW_AmpIllumina-B/README.md#5-additional-output-files))
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* counts_GLAmpSeq.tsv (output from [GL-DPPD-7104-B step 5g](https://github.com/nasa/GeneLab_Data_Processing/blob/master/Amplicon/Illumina/Pipeline_GL-DPPD-7104_Versions/GL-DPPD-7104-B.md#5g-generating-and-writing-standard-outputs))
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* taxonomy_GLAmpSeq.tsv (output from [GL-DPPD-7104-B step 5g](https://github.com/nasa/GeneLab_Data_Processing/blob/master/Amplicon/Illumina/Pipeline_GL-DPPD-7104_Versions/GL-DPPD-7104-B.md#5g-generating-and-writing-standard-outputs))
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**Output Data:**
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***dendrogram_by_group{assay_suffix}.png** (dendrogram of euclidean distance - based hierarchical clustering of the samples, colored by experimental groups)
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***rarefaction_curves{assay_suffix}.png** (Rarefaction curves plot for all samples)
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***richness_and_diversity_estimates_by_sample{assay_suffix}.png** (Richness and diversity estimates plot for all samples)
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***richness_and_diversity_estimates_by_group{assay_suffix}.png** (Richness and diversity estimates plot for all groups)
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***relative_phyla{assay_suffix}.png** (taxonomic summaries plot based on phyla, for all samples)
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***relative_classes{assay_suffix}.png** (taxonomic summaries plot based on class, for all samples)
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***samplewise_phyla{assay_suffix}.png** (taxonomic summaries plot based on phyla, for all samples)
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***samplewise_classes{assay_suffix}.png** (taxonomic summaries plot based on class, for all samples)
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***PCoA_w_labels{assay_suffix}.png** (principle Coordinates Analysis plot of VST transformed ASV counts, with sample labels)
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***PCoA_without_labels{assay_suffix}.png** (principle Coordinates Analysis plot of VST transformed ASV counts, without sample labels)
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***normalized_counts{assay_suffix}.tsv** (size factor normalized ASV counts table)
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***group1_vs_group2.csv** (differential abundance tables for all pairwise contrasts of groups)
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***volcano_group1_vs_group2.png** (volcano plots for all pairwise contrasts of groups)
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