You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Below is a description of each subworkflow and the additional output files generated that are not already indicated in the [GL-DPPD-7112 pipeline document](../../Pipeline_GL-DPPD-7112_Versions/GL-DPPD-7112.md):
19
18
20
19
1.**Analysis Staging Subworkflow**
21
20
@@ -91,16 +90,12 @@ We recommend installing Singularity on a system wide level as per the associated
91
90
92
91
### 2. Download the Workflow Files
93
92
94
-
All files required for utilizing the NF_MAAgilent1ch GeneLab workflow for processing Agilent 1 Channel Microarray data are in the [workflow_code](workflow_code) directory. To get a
95
-
copy of latest NF_MAAgilent1ch version on to your system, the code can be downloaded as a zip file from the release page then unzipped after downloading by running the following commands:
93
+
All files required for utilizing the NF_MAAgilent1ch GeneLab workflow for processing Agilent 1 Channel Microarray data are in the [workflow_code](workflow_code) directory. To get a copy of latest NF_MAAgilent1ch version on to your system, the code can be downloaded as a zip file from the release page then unzipped after downloading by running the following commands:
While in the location containing the `NF_MAAgilent1ch_1.0.2
113
-
` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_MAAgilent1ch workflow:
107
+
While in the location containing the `NF_MAAgilent1ch_1.0.2` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_MAAgilent1ch workflow:
114
108
> Note: Nextflow commands use both single hyphen arguments (e.g. -help) that denote general nextflow arguments and double hyphen arguments (e.g. --ensemblVersion) that denote workflow specific parameters. Take care to use the proper number of hyphens for each argument.
115
109
116
110
<br>
117
111
118
112
#### 3a. Approach 1: Run the workflow on a GeneLab Agilent 1 Channel Microarray dataset
119
113
120
114
```bash
121
-
nextflow run NF_MAAgilent1ch_1.0.2
122
-
/main.nf \
115
+
nextflow run NF_MAAgilent1ch_1.0.2/main.nf \
123
116
-profile singularity \
124
117
--osdAccession OSD-548 \
125
118
--gldsAccession GLDS-548
@@ -132,8 +125,7 @@ nextflow run NF_MAAgilent1ch_1.0.2
132
125
> Note: Specifications for creating a runsheet manually are described [here](examples/runsheet/README.md).
133
126
134
127
```bash
135
-
nextflow run NF_MAAgilent1ch_1.0.2
136
-
/main.nf \
128
+
nextflow run NF_MAAgilent1ch_1.0.2/main.nf \
137
129
-profile singularity \
138
130
--runsheetPath </path/to/runsheet>
139
131
```
@@ -142,8 +134,7 @@ nextflow run NF_MAAgilent1ch_1.0.2
142
134
143
135
**Required Parameters For All Approaches:**
144
136
145
-
* `NF_MAAgilent1ch_1.0.2
146
-
/main.nf` - Instructs Nextflow to run the NF_MAAgilent1ch workflow
137
+
* `NF_MAAgilent1ch_1.0.2/main.nf` - Instructs Nextflow to run the NF_MAAgilent1ch workflow
147
138
148
139
* `-profile` - Specifies the configuration profile(s) to load, `singularity` instructs Nextflow to setup and use singularity for all software called in the workflow
149
140
@@ -175,8 +166,7 @@ nextflow run NF_MAAgilent1ch_1.0.2
175
166
All parameters listed above and additional optional arguments for the NF_MAAgilent1ch workflow, including debug related options that may not be immediately useful for most users, can be viewed by running the following command:
176
167
177
168
```bash
178
-
nextflow run NF_MAAgilent1ch_1.0.2
179
-
/main.nf --help
169
+
nextflow run NF_MAAgilent1ch_1.0.2/main.nf --help
180
170
```
181
171
182
172
See `nextflow run -h` and [Nextflow's CLI run command documentation](https://nextflow.io/docs/latest/cli.html#run) for more options and details common to all nextflow workflows.
@@ -190,8 +180,7 @@ See `nextflow run -h` and [Nextflow's CLI run command documentation](https://nex
190
180
All R code steps and output are rendered within a Quarto document yielding the following:
191
181
192
182
- Output:
193
-
- NF_MAAgilent1ch_1.0.2
194
-
.html (html report containing executed code and output including QA plots)
183
+
- NF_MAAgilent1ch_1.0.2.html (html report containing executed code and output including QA plots)
195
184
196
185
197
186
The outputs from the Analysis Staging and V&V Pipeline Subworkflows are described below:
0 commit comments