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Merge pull request #90 from cyouh95/DEV_NF_MAAffymetrix
NF_MAAffymetrix: Update workflow version from 1.0.3 to 1.0.4
Fix cache location issues that arose in `quarto render` when using Nextflow v.23.10.1 and increased timeout for file download
- Fix cache location issues that arose in `quarto render` when using Nextflow v.23.10.1 ([#82](https://github.com/nasa/GeneLab_Data_Processing/issues/82))
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- Increase timeout that caused incomplete file download in `read.celfiles()` ([#86](https://github.com/nasa/GeneLab_Data_Processing/issues/86))
Copy file name to clipboardExpand all lines: Microarray/Affymetrix/Workflow_Documentation/NF_MAAffymetrix/README.md
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@@ -97,9 +97,9 @@ All files required for utilizing the NF_MAAffymetrix GeneLab workflow for proces
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copy of latest NF_MAAffymetrix version on to your system, the code can be downloaded as a zip file from the release page then unzipped after downloading by running the following commands:
While in the location containing the `NF_MAAffymetrix_1.0.3` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_MAAffymetrix workflow:
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While in the location containing the `NF_MAAffymetrix_1.0.4` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_MAAffymetrix workflow:
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> Note: Nextflow commands use both single hyphen arguments (e.g. -help) that denote general nextflow arguments and double hyphen arguments (e.g. --ensemblVersion) that denote workflow specific parameters. Take care to use the proper number of hyphens for each argument.
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<br>
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#### 3a. Approach 1: Run the workflow on a GeneLab Affymetrix Microarray dataset
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```bash
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nextflow run NF_MAAffymetrix_1.0.3/main.nf \
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nextflow run NF_MAAffymetrix_1.0.4/main.nf \
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-profile singularity \
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--osdAccession OSD-266 \
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--gldsAccession GLDS-266
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> Note: Specifications for creating a runsheet manually are described [here](examples/runsheet/README.md).
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```bash
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nextflow run NF_MAAffymetrix_1.0.3/main.nf \
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nextflow run NF_MAAffymetrix_1.0.4/main.nf \
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-profile singularity \
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--runsheetPath </path/to/runsheet>
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```
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> Note: Specifications for the ISA Tab Archive format can be found [here](https://isa-specs.readthedocs.io/en/latest/isatab.html).
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```bash
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nextflow run NF_MAAffymetrix_1.0.3/main.nf \
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nextflow run NF_MAAffymetrix_1.0.4/main.nf \
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-profile singularity \
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--isaArchivePath </path/to/isaArchive>
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```
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**Required Parameters For All Approaches:**
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* `NF_MAAffymetrix_1.0.3/main.nf` - Instructs Nextflow to run the NF_MAAffymetrix workflow
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* `NF_MAAffymetrix_1.0.4/main.nf` - Instructs Nextflow to run the NF_MAAffymetrix workflow
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* `-profile` - Specifies the configuration profile(s) to load, `singularity` instructs Nextflow to setup and use singularity for all software called in the workflow
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All parameters listed above and additional optional arguments for the NF_MAAffymetrix workflow, including debug related options that may not be immediately useful for most users, can be viewed by running the following command:
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```bash
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nextflow run NF_MAAffymetrix_1.0.3/main.nf --help
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nextflow run NF_MAAffymetrix_1.0.4/main.nf --help
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```
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See `nextflow run -h` and [Nextflow's CLI run command documentation](https://nextflow.io/docs/latest/cli.html#run) for more options and details common to all nextflow workflows.
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All R code steps and output are rendered within a Quarto document yielding the following:
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- Output:
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- NF_MAAffymetrix_1.0.3.html (html report containing executed code and output including QA plots)
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- NF_MAAffymetrix_1.0.4.html (html report containing executed code and output including QA plots)
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The outputs from the Analysis Staging and V&V Pipeline Subworkflows are described below:
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