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4 | 4 |
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5 | 5 | ### Implementation Tools <!-- omit in toc -->
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6 | 6 |
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7 |
| -The current GeneLab RNAseq consensus processing pipeline (RCP), [GL-DPPD-7101-G](../../Pipeline_GL-DPPD-7101_Versions/GL-DPPD-7101-G.md), is implemented as a [Nextflow](https://nextflow.io/) DSL2 workflow and utilizes [Singularity](https://docs.sylabs.io/guides/3.10/user-guide/introduction.html) to run all tools in containers. This workflow (NF_RCP-F) is run using the command line interface (CLI) of any unix-based system. While knowledge of creating workflows in Nextflow is not required to run the workflow as is, [the Nextflow documentation](https://nextflow.io/docs/latest/index.html) is a useful resource for users who want to modify and/or extend this workflow. |
| 7 | +The current GeneLab RNAseq consensus processing pipeline (RCP), [GL-DPPD-7101-G](../../Pipeline_GL-DPPD-7101_Versions/GL-DPPD-7101-G.md), is implemented as a [Nextflow](https://nextflow.io/) DSL2 workflow and utilizes [Singularity](https://docs.sylabs.io/guides/3.10/user-guide/introduction.html) to run all tools in containers. This workflow (NF_RCP-G) is run using the command line interface (CLI) of any unix-based system. While knowledge of creating workflows in Nextflow is not required to run the workflow as is, [the Nextflow documentation](https://nextflow.io/docs/latest/index.html) is a useful resource for users who want to modify and/or extend this workflow. |
8 | 8 |
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9 | 9 | ### Workflow & Subworkflows <!-- omit in toc -->
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10 | 10 |
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@@ -97,8 +97,8 @@ We recommend installing Singularity on a system wide level as per the associated
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97 | 97 |
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98 | 98 | ### 2. Download the Workflow Files
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99 | 99 |
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100 |
| -All files required for utilizing the NF_RCP-F GeneLab workflow for processing RNAseq data are in the [workflow_code](workflow_code) directory. To get a |
101 |
| -copy of latest NF_RCP-F version on to your system, the code can be downloaded as a zip file from the release page then unzipped after downloading by running the following commands: |
| 100 | +All files required for utilizing the NF_RCP-G GeneLab workflow for processing RNAseq data are in the [workflow_code](workflow_code) directory. To get a |
| 101 | +copy of latest NF_RCP-G version on to your system, the code can be downloaded as a zip file from the release page then unzipped after downloading by running the following commands: |
102 | 102 |
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103 | 103 | ```bash
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104 | 104 | wget https://github.com/nasa/GeneLab_Data_Processing/releases/download/NF_RCP-G_2.0.0/NF_RCP-G_2.0.0.zip
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@@ -134,7 +134,7 @@ export NXF_SINGULARITY_CACHEDIR=$(pwd)/singularity
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134 | 134 |
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135 | 135 | ### 4. Run the Workflow
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136 | 136 |
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137 |
| -While in the location containing the `NF_RCP-G_2.0.0` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_RCP-F workflow: |
| 137 | +While in the location containing the `NF_RCP-G_2.0.0` directory that was downloaded in [step 2](#2-download-the-workflow-files), you are now able to run the workflow. Below are three examples of how to run the NF_RCP-G workflow: |
138 | 138 | > Note: Nextflow commands use both single hyphen arguments (e.g. -help) that denote general nextflow arguments and double hyphen arguments (e.g. --ensemblVersion) that denote workflow specific parameters. Take care to use the proper number of hyphens for each argument.
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139 | 139 |
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140 | 140 | <br>
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@@ -180,7 +180,7 @@ nextflow run NF_RCP-G_2.0.0/main.nf \
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180 | 180 |
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181 | 181 | **Required Parameters For All Approaches:**
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182 | 182 |
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183 |
| -* `NF_RCP-G_2.0.0/main.nf` - Instructs Nextflow to run the NF_RCP-F workflow |
| 183 | +* `NF_RCP-G_2.0.0/main.nf` - Instructs Nextflow to run the NF_RCP-G workflow |
184 | 184 |
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185 | 185 | * `-profile` - Specifies the configuration profile(s) to load, `singularity` instructs Nextflow to setup and use singularity for all software called in the workflow
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186 | 186 |
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@@ -242,7 +242,7 @@ See `nextflow run -h` and [Nextflow's CLI run command documentation](https://nex
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242 | 242 | ### 5. Additional Output Files
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243 | 243 |
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244 | 244 | The outputs from the Analysis Staging and V&V Pipeline Subworkflows are described below:
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245 |
| -> Note: The outputs from the RNAseq Consensus Pipeline Subworkflow are documented in the [GL-DPPD-7101-F](../../Pipeline_GL-DPPD-7101_Versions/GL-DPPD-7101-F.md) processing protocol. |
| 245 | +> Note: The outputs from the RNAseq Consensus Pipeline Subworkflow are documented in the [GL-DPPD-7101-G](../../Pipeline_GL-DPPD-7101_Versions/GL-DPPD-7101-G.md) processing protocol. |
246 | 246 |
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247 | 247 | **Analysis Staging Subworkflow**
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248 | 248 |
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