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Copy file name to clipboardExpand all lines: GeneLab_Reference_Annotations/Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A_annotations.csv
SALTY,Salmonella enterica,serovar Typhimurium str. LT2,,ncbi,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/006/945/GCF_000006945.2_ASM694v2/GCF_000006945.2_ASM694v2_genomic.fna.gz,https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/006/945/GCF_000006945.2_ASM694v2/GCF_000006945.2_ASM694v2_genomic.gtf.gz,99287,org.SentericaserovarTyphimuriumstrLT2.eg.db,https://figshare.com/ndownloader/files/48354385,https://figshare.com/ndownloader/files/48354391
Copy file name to clipboardExpand all lines: GeneLab_Reference_Annotations/Workflow_Documentation/GL_RefAnnotTable-A/CHANGELOG.md
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- Plants: Ensembl plants release 59
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- Bacteria: Ensembl bacteria release 59
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- Removed org.EcK12.eg.db and replaced it with a locally created annotations database, as it is no longer available on Bioconductor
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- Changed the first argument of GL-DPPD-7110-A_build-genome-annots-tab.R from the 'name' column value to the 'species' column value (e.g., 'Mus musculus' instead of 'MOUSE')
Copy file name to clipboardExpand all lines: GeneLab_Reference_Annotations/Workflow_Documentation/GL_RefAnnotTable-A/README.md
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While in the GL_RefAnnotTable workflow directory, you are now able to run the workflow. Below is an example of how to run the workflow to build an annotation table for Mus musculus (mouse):
- No input files are required. Specify the target organism using a positional command line argument. `MOUSE` is used in the example above. To see a list of all available organisms, run `Rscript GL-DPPD-7110-A_build-genome-annots-tab.R` without positional arguments. The correct argument for each organism can also be found in the 'name' column of the [GL-DPPD-7110-A_annotations.csv](../../Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A_annotations.csv)
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- No input files are required. Specify the target organism using a positional command line argument. `Mus musculus` is used in the example above. To see a list of all available organisms, run `Rscript GL-DPPD-7110-A_build-genome-annots-tab.R` without positional arguments. The correct argument for each organism can also be found in the 'species' column of the [GL-DPPD-7110-A_annotations.csv](../../Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A_annotations.csv)
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- Optional: a reference table CSV can be supplied as a second positional argument instead of using the default [GL-DPPD-7110-A_annotations.csv](../../Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A_annotations.csv)
When the workflow is run, if the reference table does not specify an annotations database for the target_organism in the `annotations` column, the `install_annotations` function, defined in the `install-org-db.R` script, will be executed. This script will locally create and install an annotations database R package using AnnotationForge. This function can also be run as a stand-alone script from the command line:
- The target organism must be specified as the first positional command line argument, `BACSU` is used in the example above. The correct argument for each organism can be found in the 'name' column of the [GL-DPPD-7110-A_annotations.csv](../../Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A_annotations.csv)
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- The target organism must be specified as the first positional command line argument, `Bacillus subtilis` is used in the example above. The correct argument for each organism can be found in the 'species' column of the [GL-DPPD-7110-A_annotations.csv](../../Pipeline_GL-DPPD-7110_Versions/GL-DPPD-7110-A/GL-DPPD-7110-A_annotations.csv)
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- The path to a local reference table must also be supplied as the second positional argument
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