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Description
Hi!
I'm trying to run TOSCA on my sample but I got the following error:
Workflow defines that rule get_genome is eligible for caching between workflows (use the --cache argument to enable this).
Workflow defines that rule get_annotation is eligible for caching between workflows (use the --cache argument to enable this).
Workflow defines that rule get_known_variation is eligible for caching between workflows (use the --cache argument to enable this).
Building DAG of jobs...
MissingInputException in rule callable in file /scratch/users/astar/ares/buinguye/Variant_Calling_Tumour_Only_Models/TOSCA/rules/calling.smk, line 1:
Missing input files for rule callable:
output: /home/users/astar/ares/buinguye/scratch/Variant_Calling_Tumour_Only_Models/TOSCA/TOSCA_Preps/qc/coverage-stats/2.bed, /home/users/astar/ares/buinguye/scratch/Variant_Calling_Tumour_Only_Models/TOSCA/TOSCA_Preps/qc/coverage-stats/2.filt.bed, /home/users/astar/ares/buinguye/scratch/Variant_Calling_Tumour_Only_Models/TOSCA/TOSCA_Preps/qc/coverage-stats/2.summary.txt, /home/users/astar/ares/buinguye/scratch/Variant_Calling_Tumour_Only_Models/TOSCA/TOSCA_Preps/qc/coverage-stats/2.final.bed
wildcards: sample=2
affected files:
/scratch/users/astar/gis/simngl/SEQC2-Kiran/Exome-Agilent_V6_Padded.bed
I prepared a meta file with the same format as example_metafile.txt in the metafile directory for one sample (the file has 2 lines only) and I only changed the metafile directory and output directory in config file. May I ask if I missed any information related to data preprocessing for TOSCA, i.e , does it require other files besides of pair-end fastq files, metafile and config file? From what I see in the error it requires other bed files or txt files.
Hope you can help clarify about this. Thank you very much!