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Michael Alonge edited this page Feb 7, 2021
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Merging and reconciling scaffolds
positional arguments:
<asm.fasta> assembly fasta file (uncompressed or bgzipped)
<scf1.agp> <scf2.agp> [...]
scaffolding AGP files
optional arguments:
-h, --help show this help message and exit
merging options:
-f FILE CSV list of (AGP file,weight) [null]
-j <skip.txt> list of query headers to leave unplaced [null]
-l INT minimum assembly sequence length [100000]
-e FLOAT minimum edge weight. NA if using Hi-C [0.0]
--gap-func STR function for merging gap lengths {'min', 'max', or 'mean'} [min]
input/output options:
-o PATH output directory [./ragtag_output]
-w overwrite intermediate files
-u add suffix to unplaced sequence headers
Hi-C options:
-b FILE Hi-C alignments in BAM format, sorted by read name [null]
-r STR CSV list of restriction enzymes/sites or 'DNase' [GATC]
-p FLOAT portion of the sequence termini to consider for links [1.0]
--list-enzymes list all available restriction enzymes/sites
Are these docs confusing or incomplete? Please open an issue and let me know.