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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_100_Blood_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_100_Tissue_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_102_Blood_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_102_Tissue_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_102_Tumor_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_104_Blood_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_104_Tissue_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_104_Tumor_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_106_Blood_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_106_Tissue_CD8_3000_GA.csv

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Test/CD8_Panel_II_channelvalues_GA_downSampled/export_Lung_106_Tumor_CD8_3000_GA.csv

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Test/CD8_bulk_markers_to_exclude.txt

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Parameter_1 :: FSC-A
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Parameter_11 :: Comp-BUV496-A :: CD3
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Parameter_13 :: Comp-BUV615-A :: CD4
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Parameter_15 :: Comp-BUV805-A :: CD8
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Parameter_18 :: Comp-BV510-A :: Aqua
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Parameter_2 :: FSC-H
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Parameter_3 :: SSC-A
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Parameter_30 :: Time
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Parameter_4 :: SSC-H

Test/Info_file_bulk_Test.xlsx

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Test/infofile.txt

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File_name Experiment_name Conditions Instrument Dosages Timepoint Individuals Date CompensationMatrix
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CD3_30000_Lung41_tumor.fcs Test Tumor FACSymphony NA 0 41 01012020 /mnt/hpcserver1_datadisk2_spuccio/SP026_dev_cytophenograph/Test_dataset/CompensationMatrix.txt
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CD3_30000_Lung41_blood.fcs Test Blood FACSymphony NA 0 41 01012020 /mnt/hpcserver1_datadisk2_spuccio/SP026_dev_cytophenograph/Test_dataset/CompensationMatrix.txt
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CD3_30000_HCL270.fcs Test Healthy FACSymphony NA 0 41 01012020 NA

environment_cytophenograph2.yml

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name: cytophenograph
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channels:
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- bpteague
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- bioconda
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- conda-forge
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- defaults
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dependencies:
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- _libgcc_mutex=0.1=conda_forge
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- _openmp_mutex=4.5=1_llvm
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- aniso8601=8.1.0=pyhd3deb0d_0
11+
- anndata=0.6.22.post1=py_0
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- asn1crypto=1.4.0=pyh9f0ad1d_0
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- atk=2.36.0=ha770c72_4
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- atk-1.0=2.36.0=h3371d22_4
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- blas=2.108=openblas
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- blas-devel=3.9.0=8_openblas
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- boto3=1.17.4=pyhd8ed1ab_0
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- botocore=1.20.4=pyhd8ed1ab_0
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- brotli-python=1.0.9=py37hcd2ae1e_4
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- brotlipy=0.7.0=py37h5e8e339_1001
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- bzip2=1.0.8=h7f98852_4
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- ca-certificates=2020.12.5=ha878542_0
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- cairo=1.16.0=h18b612c_1001
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- cellxgene=0.16.6=py_0
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- certifi=2020.12.5=py37h89c1867_1
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- cffi=1.14.4=py37h11fe52a_0
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- chardet=4.0.0=py37h89c1867_1
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- click=7.1.2=pyh9f0ad1d_0
29+
- cryptography=2.5=py37hb7f436b_1
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- curl=7.64.0=h646f8bb_0
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- cycler=0.10.0=py_2
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- dbus=1.13.6=hfdff14a_1
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- expat=2.2.10=h9c3ff4c_0
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- fastobo=0.9.3=py37hfa133b6_0
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- flask=1.1.2=pyh9f0ad1d_0
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- flask-compress=1.8.0=pyhd8ed1ab_0
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- flask-cors=3.0.8=py_0
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- flask-restful=0.3.8=py_0
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- flask-server-timing=0.1.2=pyh9f0ad1d_0
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- flask-talisman=0.7.0=py_0
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- flatten-dict=0.3.0=pyh9f0ad1d_0
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- fontconfig=2.13.1=he4413a7_1000
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- freetype=2.10.4=h0708190_1
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- fribidi=1.0.10=h36c2ea0_0
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- fsspec=0.8.5=pyhd8ed1ab_0
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- gdk-pixbuf=2.42.2=h19a9c64_1
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- gettext=0.19.8.1=hf34092f_1004
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- glib=2.66.3=h58526e2_0
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- gobject-introspection=1.66.1=py37h9019ea4_3
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- graphite2=1.3.13=h58526e2_1001
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- gstreamer=1.14.5=h36ae1b5_2
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- gtk2=2.24.32=h90f3771_0
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- gunicorn=20.0.4=py37h89c1867_3
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- h5py=2.10.0=nompi_py37h513d04c_102
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- harfbuzz=2.4.0=h37c48d4_1
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- hdf5=1.10.5=nompi_h5b725eb_1114
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- icu=58.2=hf484d3e_1000
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- idna=2.10=pyh9f0ad1d_0
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- intel-openmp=2020.2=254
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- itsdangerous=1.1.0=py_0
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- jinja2=2.11.3=pyh44b312d_0
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- jmespath=0.10.0=pyh9f0ad1d_0
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- joblib=1.0.0=pyhd8ed1ab_0
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- jpeg=9d=h36c2ea0_0
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- kiwisolver=1.3.1=py37h2527ec5_1
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- krb5=1.16.3=hc83ff2d_1000
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- lcms2=2.12=hddcbb42_0
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- libblas=3.9.0=8_openblas
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- libcblas=3.9.0=8_openblas
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- libcurl=7.64.0=h01ee5af_0
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- libcxx=11.0.1=h4bd325d_0
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- libcxxabi=11.0.1=ha770c72_0
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- libedit=3.1.20191231=he28a2e2_2
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- libffi=3.2.1=he1b5a44_1007
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- libgcc-ng=9.3.0=h2828fa1_18
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- libgfortran=3.0.0=1
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- libgfortran-ng=9.3.0=hff62375_18
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- libgfortran5=9.3.0=hff62375_18
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- libglib=2.66.3=hbe7bbb4_0
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- libiconv=1.16=h516909a_0
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- liblapack=3.9.0=8_openblas
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- liblapacke=3.9.0=8_openblas
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- libllvm10=10.0.1=he513fc3_3
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- libopenblas=0.3.12=pthreads_h4812303_1
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- libpng=1.6.37=h21135ba_2
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- libssh2=1.8.0=h1ad7b7a_1003
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- libstdcxx-ng=9.3.0=h6de172a_18
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- libtiff=4.2.0=hdc55705_0
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- libuuid=2.32.1=h7f98852_1000
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- libwebp-base=1.2.0=h7f98852_0
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- libxcb=1.13=h7f98852_1003
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- libxml2=2.9.9=h13577e0_2
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- llvm-openmp=11.0.1=h4bd325d_0
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- llvmlite=0.35.0=py37h9d7f4d0_1
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- lz4-c=1.9.3=h9c3ff4c_0
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- markupsafe=1.1.1=py37h5e8e339_3
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- matplotlib=3.3.4=py37h89c1867_0
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- matplotlib-base=3.3.4=py37h0c9df89_0
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- mkl=2020.4=h726a3e6_304
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- mkl-service=2.3.0=py37h8f50634_2
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- mkl_fft=1.2.0=py37h161383b_1
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- mkl_random=1.2.0=py37h9fdb41a_1
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- natsort=7.1.1=pyhd8ed1ab_0
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- ncurses=6.2=h58526e2_4
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- numba=0.52.0=py37hdc94413_0
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- numpy=1.20.0=py37haa41c4c_0
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- olefile=0.46=pyh9f0ad1d_1
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- openblas=0.3.12=pthreads_h04b7a96_1
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- openssl=1.0.2u=h516909a_0
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- opentsne=0.5.1=py37h6dcda5c_0
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- packaging=20.9=pyh44b312d_0
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- pandas=1.2.1=py37hdc94413_0
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- pango=1.42.4=h7062337_4
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- pathlib2=2.3.5=py37h89c1867_3
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- patsy=0.5.1=py_0
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- pcre=8.44=he1b5a44_0
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- pillow=8.1.0=py37he6b4880_1
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- pip=21.0.1=pyhd8ed1ab_0
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- pixman=0.38.0=h516909a_1003
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- pthread-stubs=0.4=h36c2ea0_1001
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- pycparser=2.20=pyh9f0ad1d_2
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- pynndescent=0.5.1=pyhd3deb0d_0
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- pyopenssl=19.0.0=py37_0
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- pyparsing=2.4.7=pyh9f0ad1d_0
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- pyqt=5.6.0=py37h13b7fb3_1008
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- pysocks=1.7.1=py37h89c1867_3
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- python=3.7.0=hd21baee_1006
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- python-dateutil=2.8.1=py_0
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- python-flatbuffers=1.12=pyhd8ed1ab_0
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- python_abi=3.7=1_cp37m
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- pytz=2021.1=pyhd8ed1ab_0
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- pyyaml=5.4.1=py37h5e8e339_0
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- qt=5.6.2=hce4f676_1013
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- readline=7.0=hf8c457e_1001
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- requests=2.25.1=pyhd3deb0d_0
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- s3fs=0.4.2=py_0
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- s3transfer=0.3.4=pyhd8ed1ab_0
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- scikit-learn=0.24.1=py37h69acf81_0
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- seaborn=0.11.1=hd8ed1ab_1
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- seaborn-base=0.11.1=pyhd8ed1ab_1
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- setuptools=49.6.0=py37h89c1867_3
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- sip=4.18.1=py37hf484d3e_1000
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- six=1.15.0=pyh9f0ad1d_0
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- sqlalchemy=1.3.23=py37h5e8e339_0
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- sqlite=3.28.0=h8b20d00_0
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- statsmodels=0.12.2=py37h902c9e0_0
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- tbb=2020.2=h4bd325d_3
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- threadpoolctl=2.1.0=pyh5ca1d4c_0
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- tiledb=2.2.3=h91fcb0e_0
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- tiledb-py=0.8.2=py37h901d765_0
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- tk=8.6.10=h21135ba_1
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- tornado=6.1=py37h5e8e339_1
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- umap-learn=0.5.0=py37h89c1867_0
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- urllib3=1.26.3=pyhd8ed1ab_0
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- werkzeug=1.0.1=pyh9f0ad1d_0
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- wheel=0.36.2=pyhd3deb0d_0
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- xorg-kbproto=1.0.7=h7f98852_1002
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- xorg-libice=1.0.10=h516909a_0
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- xorg-libsm=1.2.3=h84519dc_1000
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- xorg-libx11=1.6.12=h516909a_0
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- xorg-libxau=1.0.9=h7f98852_0
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- xorg-libxdmcp=1.1.3=h7f98852_0
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- xorg-libxext=1.3.4=h516909a_0
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- xorg-libxrender=0.9.10=h516909a_1002
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- xorg-libxt=1.2.1=h7f98852_0
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- xorg-renderproto=0.11.1=h14c3975_1002
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- xorg-xextproto=7.3.0=h7f98852_1002
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- xorg-xproto=7.0.31=h7f98852_1007
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- xz=5.2.5=h516909a_1
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- yaml=0.2.5=h516909a_0
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- zlib=1.2.11=h516909a_1010
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- zstd=1.4.8=ha95c52a_1
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- pip:
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- colorlog==4.7.2
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- leidenalg==0.8.3
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- parc==0.31
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- scipy==1.4.1
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prefix: /home/spuccio/miniconda3/envs/cytophenograph

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